Phospho-Zap70 (Tyr319)/Syk (Tyr352) (A3) rabbit mAb

• Catalog Number: orb1946288
• Category: Antibodies
• Description: Phospho-Zap70 (Tyr319)/Syk (Tyr352) (A3) rabbit mAb
• Clonality: Monoclonal
• Isotype: Rabbit IgGk
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Concentration: 0.5 mg/ml
• Buffer/Preservatives: 1X PBS, 0.02% NaN3, 50% Glycerol, 0.1% rAlbumin
• Purification: Protein A+G
• Immunogen: A synthetic phospho-peptide corresponding to residues surrounding Tyr319/Tyr352 of human phospho Zap70/Syk.
• UniProt ID: P43405, P43403
• Tested applications: FC
• Dilution range: 1µg/mL – 0.001µg/mL. It is recommended that the reagent be titrated for optimal performance for each application. See product image legends for additional information.
• Cross Reactivity: Predicted to work with mouse, rat and other homologues.
• Clone Number: Zap70Y319-A3
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Tyrosine-protein kinase ZAP-70 , 70 kDa zeta-chain
• Note: For research use only

ZapY319-A3 recognizes basal phosphorylation levels in mouse cells. Flow cytometric analysis of L929 cells secondary antibody only (blue) or 0.1 μg/mL of isotype control Cat. #orb1946241 (orange) or of Phospho-Zap70 (Tyr319) /Syk (Tyr352) antibody ZapY319-A3 (green) Cat. #orb1946288.

Flow cytometric analysis of Jurkat cells secondary antibody only negative control (blue) untreated (red) treated with pervanadate (green) treated + blocked with non-phospho-peptide (violet) or treated + blocked with phospho-peptide (brown) using Phospho-Zap70 (Tyr319) /Syk (Tyr352) antibody ZapY319-A3 (0.05 ?μg/mL) Cat. #orb1946288.

Flow cytometric analysis of Jurkat cells secondary antibody only negative control (blue) or untreated (red) or treated with H2O2 (green) using 0.01 μg/mL Phospho-Zap70 (Y319) /Syk (Y352) antibody ZapY319-A3 (Cat. #orb1946288) or Company C antibody at 0.2 μg/mL (manufacturer's recommended concentration).

Flow cytometric analysis of Jurkat cells, untreated (red) or treated with pervanadate (green) using Phospho-Zap70 (Tyr319) /Syk (Tyr352) A3 antibody Zap70Y319-A3 at 0.01 μg/mL, Cat.#orb1946287 or concentration matched isotope (KLH-G9) for untreated cells (gray) or treated with pervandate (blue) Cat.#orb1946241 #orb1946019.