Phospho-TrkB (Tyr817) Recombinant Rabbit Monoclonal Antibody

SKU: orb559172

Description

Phospho-TrkB (Tyr817) Recombinant Rabbit Monoclonal Antibody

Images & Validation

• Tested Applications: ICC, IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:50-200, IHC-F=1:50-200, ICC/IF=1:50-200, IF=1:50-200
• Reactivity: Mouse, Rat
• Predicted Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Recombinant
• Isotype: IgG
• Clone No.: 2A1
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from human NTRK2 around the phosphorylation site of Tyr817 PV(p-Y)LD
• Target: NTRK2
• Molecular Weight: 90 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

TrkB(Y817); TrkB(phospho Y817); p-TrkB(phospho Y817); TrkB(phospho-Tyr817); TrkB(phospho Tyr817); TrkB(phospho-Y817); p-TrkB(Tyr817); AI848316; BDNF tropomyosine receptor kinase B; BDNF/NT 3 growth factors receptor; BDNF/NT-3 growth factors receptor; Brain derived neurotrophic factor receptor; C030027L06Rik; EC 2.7.10.1; GP145 TrkB; GP145-TrkB; GP145-TrkB/GP95-TrkB; GP95 TrkB; Neurotrophic tyrosine kinase receptor type 2; Neurotrophin receptor tyrosine kinase type 2; NTRK 2; Ntrk2; NTRK2_HUMAN; Obesity, hyperphagia, and developmental delay, included; RATTRKB1; Trk B; Trk-B; TrkB tyrosine kinase; TRKB1; Tropomyosin related kinase B; tyrosine kinase receptor B; Tyrosine receptor kinase B.

Paraformaldehyde-fixed, paraffin embedded (mouse kidney), Antigen retrieval by microwaving in EDTA buffer (Ph8.0) for 5 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Phospho-TrkB (Tyr817)) Monoclonal Antibody, Unconjugated (orb559172) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Lane 1: Hela cell lysate, Lane 2: Jurkat cell lysate, Primary: Anti-Phospho-TrkB (Tyr817) (orb559172) at 1:500 dilution, Secondary: Goat Anti-Rabbit IgG - HRP at 1:5000 dilution, Predicted band size: 90 kD, Observed band size: 70 kD.

Sample: Lane 1: Mouse Cerebrum tissue lysates, Lane 2: Mouse Cerebellum tissue lysates, Lane 3: Mouse Spinal cord tissue lysates, Lane 4: Rat Cerebrum tissue lysates, Lane 5: Rat Cerebellum tissue lysates, Primary: Anti-Phospho-TrkB (Tyr817) (orb559172) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 90 kDa, Observed band size: 115 kDa.

Quick Database Links

Gene Symbol

NTRK2

UniProt

Q16620