Phospho-TrkB (Tyr817) Recombinant Rabbit Monoclonal Antibody

• Catalog Number: orb559172
• Category: Antibodies
• Description: Phospho-TrkB (Tyr817) Recombinant Rabbit Monoclonal Antibody
• Target: NTRK2
• Clonality: Recombinant
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Mouse, Rat
• Predicted Reactivity: Human
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Purification: Affinity purified by Protein A
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from human NTRK2 around the phosphorylation site of Tyr817 PV(p-Y)LD
• UniProt ID: Q16620
• MW: 90 kDa
• Tested applications: ICC, IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:50-200, IHC-F=1:50-200, ICC/IF=1:50-200, IF=1:50-200
• Antibody Type: Primary Antibody
• Clone Number: 2A1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: TrkB(Y817); TrkB(phospho Y817); p-TrkB(phospho Y81
• Research Area: Cancer Research, Cell Biology, Cellular Metabolisn
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Paraformaldehyde-fixed, paraffin embedded (mouse kidney), Antigen retrieval by microwaving in EDTA buffer (Ph8.0) for 5 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Phospho-TrkB (Tyr817)) Monoclonal Antibody, Unconjugated (orb559172) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Lane 1: Hela cell lysate, Lane 2: Jurkat cell lysate, Primary: Anti-Phospho-TrkB (Tyr817) (orb559172) at 1:500 dilution, Secondary: Goat Anti-Rabbit IgG - HRP at 1:5000 dilution, Predicted band size: 90 kD, Observed band size: 70 kD.

Sample: Lane 1: Mouse Cerebrum tissue lysates, Lane 2: Mouse Cerebellum tissue lysates, Lane 3: Mouse Spinal cord tissue lysates, Lane 4: Rat Cerebrum tissue lysates, Lane 5: Rat Cerebellum tissue lysates, Primary: Anti-Phospho-TrkB (Tyr817) (orb559172) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 90 kDa, Observed band size: 115 kDa.