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Phospho-TrkB (Tyr817) Recombinant Rabbit Monoclonal Antibody
Catalog Number: orb559172
| Catalog Number | orb559172 |
|---|---|
| Category | Antibodies |
| Description | Phospho-TrkB (Tyr817) Recombinant Rabbit Monoclonal Antibody |
| Species/Host | Rabbit |
| Clonality | Recombinant |
| Clone Number | 2A1 |
| Tested applications | ICC, IF, IHC-Fr, IHC-P, WB |
| Predicted Reactivity | Mouse, Rat |
| Reactivity | Human, Mouse, Rat |
| Isotype | IgG |
| Immunogen | KLH conjugated Synthesised phosphopeptide derived from human NTRK2 around the phosphorylation site of Tyr817 PV(p-Y)LD |
| Antibody Type | Recombinant Antibody |
| Concentration | 1mg/ml |
| Dilution range | WB=1:500-2000, IHC-P=1:50-200, IHC-F=1:50-200, ICC/IF=1:50-200, IF=1:50-200 |
| Form/Appearance | Liquid |
| Conjugation | Unconjugated |
| MW | 90 kDa |
| Target | NTRK2 |
| UniProt ID | Q16620 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Alternative names | TrkB(Y817); TrkB(phospho Y817); p-TrkB(phospho Y81 Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
Paraformaldehyde-fixed, paraffin embedded (mouse kidney), Antigen retrieval by microwaving in EDTA buffer (Ph8.0) for 5 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Phospho-TrkB (Tyr817)) Monoclonal Antibody, Unconjugated (orb559172) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Sample: Lane 1: Hela cell lysate, Lane 2: Jurkat cell lysate, Primary: Anti-Phospho-TrkB (Tyr817) (orb559172) at 1:500 dilution, Secondary: Goat Anti-Rabbit IgG - HRP at 1:5000 dilution, Predicted band size: 90 kD, Observed band size: 70 kD.
Sample: Lane 1: Mouse Cerebrum tissue lysates, Lane 2: Mouse Cerebellum tissue lysates, Lane 3: Mouse Spinal cord tissue lysates, Lane 4: Rat Cerebrum tissue lysates, Lane 5: Rat Cerebellum tissue lysates, Primary: Anti-Phospho-TrkB (Tyr817) (orb559172) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 90 kDa, Observed band size: 115 kDa.
