Phospho-TBK1 (Ser172) Recombinant Rabbit Monoclonal Antibody

SKU: orb1499322

Description

Phospho-TBK1 (Ser172) Recombinant Rabbit Monoclonal Antibody

Research Area

Kinases, NF-kB Signalling Pathway

Images & Validation

• Tested Applications: WB
• Dilution Range: WB=1:500-2000
• Reactivity: Human
• Predicted Reactivity: Human, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Recombinant
• Isotype: IgG
• Clone No.: R7D3
• Immunogen: A synthesized peptide derived from human TBK1 around the phosphorylation site of S172 QFV-pS-LYG
• Target: TBK1
• Molecular Weight: 84 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

EC 2.7.11.1; FLJ11330; NAK; NF kappa B activating kinase; NF kB activating kinase; NF-kappa-B-activating kinase; Serine/threonine protein kinase TBK 1; Serine/threonine protein kinase TBK1; Serine/threonine-protein kinase TBK1; T2K; TANK binding kinase 1; TANK-binding kinase 1; TBK 1; TBK1_HUMAN.

Blocking buffer: 5% NFDM/TBST, Primary ab Dilution: 1:2000, Primary ab incubation condition: 2 hours at room temperature, Secondary ab: Goat Anti-Rabbit IgG H&L (HRP), Lysate: (-): C6, (+): C6 + Calyculin A (100nM, 30 min), Protein loading quantity: 20 µg, Exposure time: 60 s, Predicted MW: 84 kDa, Observed MW: 84 kDa.

Blocking buffer: 5% NFDM/TBST, Primary ab Dilution: 1:2000, Primary ab incubation condition: 2 hours at room temperature, Secondary ab: Goat Anti-Rabbit IgG H&L (HRP), Lysate: (-): HeLa, (+): HeLa + Okadaic A+, Calyculin A (100 nM, 60min), Protein loading quantity: 20 µg, Exposure time: 60 s, Predicted MW: 84 kDa, Observed MW: 84 kDa.

Quick Database Links

Gene Symbol

TBK1

UniProt

Q9UHD2