Phospho-STAT5a(Y694) Antibody

SKU: orb1931216

Description

Affinity Purified Rabbit Polyclonal Antibody (Pab)

Research Area

Signal Transduction

Images & Validation

• Tested Applications: IF, IHC-P, WB
• Dilution Range: IF - 1:200, WB - 1:1000, IHC-P - 1:50-100
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: BTD7178
• Immunogen: This STAT5a Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding Y694 of human STAT5a.
• Target: STAT5A
• Molecular Weight: 90647 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Signal transducer and activator of transcription 5A, STAT5A, STAT5

Fluorescent confocal image of an SY5Y cell stained with Phospho-STAT5a antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min), then incubated with Phospho-STAT5a primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1 h).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (5.25 μM, 25 min). Nuclei were counterstained with Hoechst 33342 (blue) (10 µg/ml, 3 min) (not shown). Phospho-STAT5a immunoreactivity is localized exclusively to the nucleus.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Western blot analysis of anti-Phospho-STAT5 Pab in HepG2 cell line lysate (35 ug/lane). Phospho-STAT5 (arrow) was detected using the purified Pab.

Quick Database Links

Gene Symbol

STAT5A

UniProt

P42229

RefSeq (Protein):NP_003143.2