Phospho-Stat3 (Tyr705) rabbit mAb Antibody

SKU: orb1946365

Description

Considered an oncogene, Stat3 is constitutively active in 70% of solid and hematological tumors, including leukemia, lymphoma, and multiple myeloma. The IL-6-Jak-Stat3 pathway can mediate cancer inflammation through both mutation of key regulatory genes and environmental stressors. Attempts at directly targeting Stat3 in cancer therapy have focused on the development of phosphopeptides and mimics that interact with the phospho-tyrosine-SH2 domain of Stat3 in an effort to destabilize active dimers and prevent DNA binding. Stat3 is primarily phosphorylated at Tyr705 upon activation of the Jak-Stat3 pathway. Secondary phosphorylation at Tyr727 at the C-terminus is thought to occur after Tyr705 phosphorylation. However, studies in melanoma have shown constitutive phosphorylation at Tyr727 that promotes survival of these cancerous cells.

Research Area

Cancer Biology

Images & Validation

• Tested Applications: FC
• Dilution Range: 1µg/mL - 0.001µg/mL. It is recommended that the reagent be titrated for optimal performance for each application. See product image legends for additional information.
• Reactivity: Human, Mouse

Key Properties

• Host: Rabbit
• Clonality: Monoclonal
• Isotype: Rabbit IgG1k
• Clone No.: BD34
• Immunogen: A synthetic phospho-peptide corresponding to residues surrounding Tyr705 of human phospho Stat3
• Purification: Protein A+G
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 1X PBS, 0.02% NaN3, 50% Glycerol, 0.1% rAlbumin
• Concentration: 0.5 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Signal transducer and activator of transcription 3, STAT3, Acute-phase response factor, APRF

Flow cytometric analysis of Jurkat cells secondary antibody only negative control (blue) untreated (red) treated with IFNα IL-4 and pervanadate (green) treated + blocked with phospho-peptide (brown) using Phospho-Stat3 (Tyr705) antibody Stat3Y705-B12 (5 ng/mL) Cat. #orb1946365.

Stat3Y705-B12 recognizes basal phosphorylation levels in mouse cells. Flow cytometric analysis of L929 cells secondary antibody only (blue) or 0.1 μg/mL of isotype control Cat. #orb1946241 (orange) or of Phospho-Stat3 (Tyr705) antibody Stat3Y705-B12 (green) Cat. #orb1946365.

Flow cytometric analysis of Jurkat cells secondary antibody only negative control (blue) or untreated (red) or treated with IFNα IL-4 and pervanadate (green) using Phospho-Stat3 (Tyr705) antibody Stat3Y705-B12 (5 ng/mL). Cat. #orb1946365.

Flow cytometric analysis of Jurkat cells secondary antibody only negative control (blue) or untreated (red) or treated with IFNα + IL-4 + pervanadate (green) using Phospho-Stat3 (Y705) antibody Stat3Y705-B12 (Cat. #orb1946365) or Company C antibody at 0.62 μg/mL (manufacturer's recommended concentration).

Quick Database Links

UniProt

P40763