Phospho-Stat1 (Ser727) (C6) rabbit mAb

• Catalog Number: orb1946146
• Category: Antibodies
• Description: Phospho-Stat1 (Ser727) (C6) rabbit mAb
• Clonality: Monoclonal
• Clone Number: Stat1S727-C6
• Tested applications: FC, WB
• Reactivity: Human
• Isotype: Rabbit IgGk
• Immunogen: A synthetic phospho-peptide corresponding to residues surrounding Ser727 of human phospho Stat1
• Concentration: 0.5 mg/ml
• Dilution range: 1µg/mL – 0.001µg/mL. It is recommended that the reagent be titrated for optimal performance for each application. See product image legends for additional information.
• Conjugation: Unconjugated
• UniProt ID: P42224
• Storage: -20°C
• Buffer/Preservatives: 1X PBS, 0.02% NaN3, 50% Glycerol, 0.1% rAlbumin
• Alternative names: Signal transducer and activator of transcription 1
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Flow cytometric analysis of U937 cells untreated (red) or treated with IFNa + IL4 + PV (green) using Phospho-Stat1 (Ser727) (C6) Rabbit mAb Stat1S727-C6 at 0.01 ug/mL #orb1946146, or concentration-matched Rabbit (G9) mAb IgG Isotype Control #orb1946241 for cells untreated (black) or treated with IFNa + IL4 + PV (blue).

Western blot analysis of U937 cell extract untreated or treated with IL-4 using 0.1 μg/mL Phospho-Stat1 (Ser727) antibody Stat1S727-C6 Cat. #orb1946146.

Western blot analysis of U937 cell extract untreated or treated with sorbitol using 0.005 μg/mL Phospho-Stat1 (Ser727) antibody Stat1S727-C6 Cat. #orb1946146 or Company C antibody at 0.04 μg/mL (manufacturer's recommended concentration) developed using the same exposure.