Phospho-Src (Tyr529) Rabbit Polyclonal Antibody

SKU: orb11413

Description

Phospho-Src (Tyr529) Rabbit Polyclonal Antibody

Research Area

Cancer Research, Proto-oncogenes, Signal Transduction

Images & Validation

• Tested Applications: ELISA, IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, ELISA=1:5000-10000
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Human, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from mouse Src around the phosphorylation site of Tyr529 YF(p-T)S
• Target: SRC
• Molecular Weight: 61 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

Src(phospho Y529); p-Src(phospho Y529); Src(phospho-Tyr529); ASV; Avian sarcoma virus; c SRC; CDNA FLJ14219 fis clone NT2RP3003800 highly similar to Rattus norvegicus tyrosine protein kinase pp60 c src mRNA; cSrc; EC 2.7.10.2; Neuronal CSRC tyrosine specific protein kinase; Neuronal SRC; Oncogene SRC; OTTHUMP00000030931; OTTHUMP00000174476; OTTHUMP00000174477; p60 Src; p60-Src; p60Src; pp60c src; pp60c-src; pp60csrc; Proto oncogene tyrosine protein kinase Src; Proto-oncogene c-Src; Proto-oncogene tyrosine-protein kinase Src; Protooncogene SRC; protooncogene SRC Rous sarcoma; Src; SRC Oncogene; SRC_MOUSE; SRC1; Tyrosine kinase pp60c src; Tyrosine protein kinase SRC 1; Tyrosine protein kinase SRC1; v src avian sarcoma(Schmidt Ruppin A2) viral oncogene homolog; V src sarcoma(Schmidt Ruppin A 2) viral oncogene homolog(avian); v src sarcoma(Schmidt Ruppin A 2) viral oncogene homolog avian; vsrc avian sarcoma(Schmidt Ruppin A2) viral oncogene homolog.

Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (phospho-Src (Tyr529)) Polyclonal Antibody, Unconjugated (orb11413) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

Sample: Lane 1: Mouse Cerebrum tissue lysates, Lane 2: Mouse Lung tissue lysates, Lane 3: Rat Cerebrum tissue lysates, Lane 4: Rat Lung tissue lysates, Lane 5: Human A431 cell lysates, Primary: Anti-phospho-Src (Tyr529) (orb11413) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 61Da, Observed band size: 60Da.

Sample: Lane 1: Normal Human Hela cell lysates, Lane 2: HeLa treated with H2O2 at 10mM for 1 h, Primary: Anti-phospho-Src (Tyr529) (orb11413) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 61Da, Observed band size: 60Da.

Quick Database Links

Gene Symbol

SRC

UniProt

P05480