Phospho-SLP-76 (Tyr128) rabbit mAb APC conjugate Antibody

SKU: orb1946243

Description

SH2 Domain-Containing Leukocyte Protein Of 76 KDa (SLP-76) is an adaptor protein that plays a role in signal transduction in T cells. Studies using a SLP-76-deficient T cell line have demonstrated that SLP-76 is required for optimal phosphorylation and activation of both PLCg1 and the Ras pathway. SLP-76 phosphorylation is mediated by Zap70 upon TCR stimulation. Within an N-terminal acidic region, SLP-76 possesses three tyrosines (Tyr113, 128, and 145), which are phosphorylated upon activation. The sterile α-motif (SAM) domain of SLP-76 drives formation of dimers and higher order oligomers. SLP-76 micro-clusters at the immunological synapse enhance signal transduction and T cell activation.

Research Area

Signal Transduction

Images & Validation

• Tested Applications: FC
• Dilution Range: For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells or 5 µL per 100 µL of staining volume. It is recommended that the reagent be titrated for optimal performance for each application.
• Reactivity: Human, Mouse

Key Properties

• Host: Rabbit
• Clonality: Monoclonal
• Isotype: Rabbit IgG1k
• Clone No.: B5H0
• Immunogen: A synthetic phospho-peptide corresponding to residues surrounding Tyr128 of human phospho SLP-76
• Purification: Protein A+G
• Conjugation: APC

Storage & Handling

• Storage: 2-8°C
• Form/Appearance: Liquid
• Buffer/Preservatives: 1X PBS, 0.09% NaN3, 0.2% rAlbumin
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Lymphocyte cytosolic protein 2, SH2 domain-containing leukocyte protein of 76 kDa, SLP76, LCP2

Flow cytometric analysis of Ramos cells unstained untreated Ramos cells negative control (blue) or stained untreated (red) or treated cells with pervanadate (green) using phospho-SLP-76 (Tyr128) antibody SLP76Y128-3F8 APC conjugate. Cat. #orb1946243.

Quick Database Links

UniProt

Q13094