Phospho-Shp2 (Tyr580) (4A2) rabbit mAb PE conjugate Antibody

SKU: orb1946267

Description

Src homology region 2 (SH2)-containing protein tyrosine phosphatase 2 (Shp2, phospho Shp2), encoded by PTPN11 gene, is a non-receptor phosphotyrosine phosphatase which is ubiquitously expressed in mammalian cells and contain one protein tyrosine phosphatase (PTP) catalytic domain and two SH2 domains. The phosphatase active site is located in the C-terminal of Shp2. Shp2 is phosphorylated by several stimulants and cytokines at Tyr580 inducing Shp2 activation. Activated Shp2 recruits Grb2 and Tyr580 phosphorylation of phospho Shp2 functions as the main binding site of Grb2, thereby activating downstream Ras in response to growth factors. In turn Grb2 controls FGFR2 phosphorylation by inhibiting receptor kinase and Shp2 phosphatase activity. Shp2 also promotes both ERK1/2 and PI3K/AKT signaling. High levels of Shp2 has been found in several cancer types including breast cancer and melanoma.

Images & Validation

• Tested Applications: FC
• Dilution Range: For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells or 5 µL per 100 µL of staining volume. It is recommended that the reagent be titrated for optimal performance for each application.
• Reactivity: Human, Mouse

Key Properties

• Host: Rabbit
• Clonality: Monoclonal
• Isotype: Rabbit IgG1k
• Clone No.: Shp2Y580-4A2
• Immunogen: A synthetic phospho-peptide corresponding to residues surrounding Tyr580 of human phospho Shp2
• Purification: Protein A+G
• Conjugation: PE

Storage & Handling

• Storage: 2-8°C
• Form/Appearance: Liquid
• Buffer/Preservatives: 1X PBS, 0.09% NaN3, 0.2% rAlbumin
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Tyrosine-protein phosphatase non-receptor type 11, Protein-tyrosine phosphatase 1D, PTP-1D, Protein-tyrosine phosphatase 2C, SH-PTP3, PTPN11, PTP2C, SHPTP2

Flow cytometric analysis of U937 cells unstained untreated cells as negative control (blue) or stained and untreated (red) or treated cells with IFNα IL4 and pervanadate (green) using phospho-Shp2 (Tyr580) antibody Shp2Y580-4A2 PE conjugate. Cat. # orb1946267

Quick Database Links

UniProt

Q06124