Phospho-SEK1/MKK4 (Ser257) (C5) rabbit mAb Antibody

• Catalog Number: orb1946068
• Category: Antibodies
• Description: Phospho-SEK1/MKK4 (Ser257) (C5) rabbit mAb Antibody
• Clonality: Monoclonal
• Clone Number: SEK1MKK4S257-C5
• Tested applications: FC, WB
• Reactivity: Human, Monkey, Mouse
• Isotype: Rabbit IgGk
• Immunogen: A synthetic phospho-peptide corresponding to residues surrounding Ser257 of human phospho SEK1/MKK4
• Concentration: 0.5 mg/ml
• Dilution range: 1µg/mL – 0.001µg/mL. It is recommended that the reagent be titrated for optimal performance for each application. See product image legends for additional information.
• Conjugation: Unconjugated
• UniProt ID: P45985
• Storage: -20°C
• Buffer/Preservatives: 1X PBS, 0.02% NaN3, 50% Glycerol, 0.1% rAlbumin
• Alternative names: Dual specificity mitogen-activated protein kinase
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Flow cytometric analysis of HEK293T cells treated with lambda phosphatase (red) or treated with UV + TPA (green) using Phospho-SEK1/MKK4 (Ser257) (C5) Rabbit mAb, at 0.1 ug/mL, SEK1MKK4S257-C5 #orb1946068, or concentration-matched Rabbit (G9) mAb IgG Isotype Control #orb1946241 for cells treated with phosphatase (black) or treated with UV + TPA (blue).

Flow cytometric analysis of 3T3 cells secondary antibody only negative control (blue) or treated with imatinib (red) or with pervanadate (green) using Phospho-SEK1/MKK4 (Ser257) antibody SEK1MKK4S257-C5 at 0.1 μg/mL. Cat. #orb1946068.

Western blot analysis of THP1 cell extract, untreated or treated with sorbitol using phospho-SEK1/MKK4 (Ser257) antibody SEK1MKK4S257-C5 at 0.05 ug/mL. Cat. #orb1946068.

Western blot analysis of COS7 cell extract, untreated or treated with UV plus TPA using phospho-SEK1/MKK4 (Ser257) antibody SEK1MKK4S257-C5 at 0.05 ug/mL. Cat. #orb1946068.

Western blot analysis of THP1 cell extract untreated or treated with sorbitol using 0.05 μg/mL Phospho-SEK1/MKK4 (Ser257) antibody SEK1MKK4S257-C5 Cat. #orb1946068 or Company C antibody at 0.01 μg/mL (manufacturer's recommended concentration) developed using the same exposure.