Phospho-SAPK/JNK (Thr183/Tyr185) (A11) rabbit mAb Antibody

SKU: orb1946073

Description

The SAPK/JNK pathway initiates apoptosis upon exposure to radiation, UV exposure, heat shock, oxidative stress, and other stressors. Upon exposure to environmental stress, the SAPK/JNK signaling pathway sequentially activates the proteins MEKK1, SEK1, SAPK, and c-Jun. Upstream activators of the SAPK/JNK cascade include ceramide, small GTP-binding proteins such as Rac1 and Cdc42Hs, Ask1, and caspases. MKK7 is also a major and direct SAPK/JNK activator in the TNFα or environmental stress signaling pathways, where its kinase activity directly phosphorylates SAPK/JNK. This relationship between MKK7 and SAPK appears to be evolutionarily conserved, as it is preserved in their Drosophila homologues, Hep and DJNK, respectively.

Images & Validation

• Tested Applications: FC
• Dilution Range: 1µg/mL - 0.001µg/mL. It is recommended that the reagent be titrated for optimal performance for each application. See product image legends for additional information.
• Reactivity: Human

Key Properties

• Host: Rabbit
• Clonality: Monoclonal
• Isotype: Rabbit IgG1k
• Clone No.: SAPKT183Y185-A11
• Immunogen: A synthetic phospho-peptide corresponding to residues surrounding Thr183/Tyr185 of human phospho SAPK/JNK
• Purification: Protein A+G
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 1X PBS, 0.02% NaN3, 50% Glycerol, 0.1% rAlbumin
• Concentration: 0.5 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Mitogen-activated protein kinase 8, MAPK8, Stress-activated protein kinase 1c, SAPK1c, c-Jun N-terminal kinase 1, JNK1, PRKM8

Peptide blocking flow cytometric analysis of 293T cells secondary antibody only negative control (light blue) or treated with K252a (red) or with UV + TPA (green) or K252a and blocked with phospho-peptide (black) or UV and blocked with phospho pepeptide (gold) or K252a and blocked with non-phospho peptide (blue) or UV and blocked with non-phospho peptide (purple) using Phospho-SAPK/JNK (Thr183/Tyr185) antibody SAPKT183Y185-A11 at 0.01 μg/mL. Cat. #orb1946073.

Flow cytometric analysis of 293T cells secondary antibody only negative control (blue) or treated with K252a (red) or with UV + TPA (green) using Phospho-SAPK/JNK (Thr183/Tyr185) antibody SAPKT183Y185-A11 at 0.01 μg/mL. Cat. #orb1946073.

Quick Database Links

UniProt

P45983