Phospho-S6 Ribosomal Protein (Ser235/236) (R3A2) rabbit mAb FITC conjugate Antibody

SKU: orb1946325

Description

Ribosomal protein S6 kinase is one of two parallel signaling pathways downstream of mTOR, with the other being 4E-BP1. mTOR phosphorylates and activates S6 kinase, which then phosphorylates ribosomal protein S6. The pathway regulates cell growth and cell cycle progression. The identified phosphorylation sites of S6 are Ser235, Ser236, Ser240, Ser244, and Ser247, which are evolutionarily conserved in higher eukaryotes. Ser236 has been proposed as the primary phosphorylation site. Studies using S6 knockin mice, where all five phosphorylation site serine residues are replaced by alanine, have provided extensive detail on S6 function. These studies support the role phosphorylated S6 plays in regulation of cell size, glucose homeostasis, and protein synthesis.

Images & Validation

• Tested Applications: FC
• Dilution Range: For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells or 5 µL per 100 µL of staining volume. It is recommended that the reagent be titrated for optimal performance for each application.
• Reactivity: Human, Mouse

Key Properties

• Host: Rabbit
• Clonality: Monoclonal
• Isotype: Rabbit IgG1k
• Clone No.: S6S235S236-R3A2
• Immunogen: A synthetic phospho-peptide corresponding to residues surrounding Ser235/236 of human phospho S6 Ribosomal Protein
• Purification: Protein A+G
• Conjugation: FITC

Storage & Handling

• Storage: 2-8°C
• Form/Appearance: Liquid
• Buffer/Preservatives: 1X PBS, 0.09% NaN3, 0.2% rAlbumin
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

40S ribosomal protein S6, Phosphoprotein NP33, Small ribosomal subunit protein eS6, RPS6

Flow cytometric analysis of U937 cells unstained U0126 and SB20350 treated cells (blue) or stained and treated with U0126 plus SB20350 (red) or treated with TPA plus calyculin A (green) using phospho-S6 ribosomal protein (Ser235/Ser236) antibody S6S235S236-R3A2 FITC conjugate Cat. #orb1946325.

Quick Database Links

UniProt

P62753