Phospho-RPS6KA1(T359) Antibody

• Catalog Number: orb1931132
• Category: Antibodies
• Description: Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB13385
• Tested applications: DOT, WB
• Predicted Reactivity: Other
• Reactivity: Human
• Isotype: Rabbit IgG
• Dilution range: WB: 1:2000, DB: 1:500
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 82723 Da
• Target: This RPS6KA1 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding T359 of human RPS6KA1.
• UniProt ID: Q15418
• NCBI: NP_002944.2, NP_001006666.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Ribosomal protein S6 kinase alpha-1, S6K-alpha-1,
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Dot blot analysis of anti-RPS6KA1-pT359 Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.

Western blot analysis of extracts from Hela cells, untreated or treated with TPA, 200nM, using phospho RPS6KA1-T359 (left) or RPS6KA1 antibody (right)