Phospho-RPS6KA1 (Ser380) Recombinant Rabbit Monoclonal Antibody

SKU: orb559128

Description

Phospho-RPS6KA1 (Ser380) Recombinant Rabbit Monoclonal Antibody

Research Area

MAPK/ERK Signalling Pathway

Images & Validation

• Tested Applications: ICC, IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:1000-5000, IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100-500, IF=1:100-500
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Mouse

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Recombinant
• Isotype: IgG
• Clone No.: 7A12
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from human RPS6KA1 around the phosphorylation site of Ser380 GF(p-S)FV
• Target: RPS6KA1
• Molecular Weight: 90 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

RSK1(S380); RPS6KA1(phospho Ser380); RPS6KA1(phospho S380); KS6A1_HUMAN; Ribosomal protein S6 kinase alpha-1; EC:2.7.11.1; MAPKAPK1A; RSK1; S6K-alpha-1; 90 kDa ribosomal protein S6 kinase 1(p90-RSK 1; p90RSK1; p90S6K); MAP kinase-activated protein kinase 1a(MAPK-activated protein kinase 1a; MAPKAP kinase 1a; MAPKAPK-1a); Ribosomal S6 kinase 1(RSK-1); ribosomal protein S6 kinase A1; RSK; HU-1; p90Rsk; MAPKAPK1; RSK1 p90;

293T cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (Phospho-RSK1 (S380)) monoclonal Antibody, Unconjugated (orb559128) 1:50, 90 minutes at 37°C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

A431 cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (Phospho-RSK1 (S380)) monoclonal Antibody, Unconjugated (orb559128) 1:50, 90 minutes at 37°C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

Hela cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (Phospho-RSK1 (S380)) monoclonal Antibody, Unconjugated (orb559128) 1:50, 90 minutes at 37°C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

Paraformaldehyde-fixed, paraffin embedded (human colon), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Phospho-RPS6KA1 (Ser380)) Monoclonal Antibody, Unconjugated (orb559128) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat testis), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Phospho-RPS6KA1 (Ser380)) Monoclonal Antibody, Unconjugated (orb559128) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Lane 1: A431 cell lysates, Primary: Anti-Phospho-RPS6KA1 (Ser380) (orb559128) at 1/1000 dilution, Secondary: Goat Anti-Rabbit IgG - HRP at 1:5000 dilution, Predicted band size: 81 kD, Observed band size: 81 kD.

Quick Database Links

Gene Symbol

RPS6KA1