You have no items in your shopping cart.
Cart summary
Item 1 of 1
Phospho-Rb (Ser807) Recombinant Rabbit Monoclonal Antibody
Catalog Number: orb559130
| Catalog Number | orb559130 |
|---|---|
| Category | Antibodies |
| Description | Phospho-Rb (Ser807) Recombinant Rabbit Monoclonal Antibody |
| Target | RB1 |
| Clonality | Recombinant |
| Species/Host | Rabbit |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Reactivity | Human |
| Predicted Reactivity | Mouse, Rat |
| Form/Appearance | Liquid |
| Concentration | 1mg/ml |
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Immunogen | KLH conjugated Synthesised phosphopeptide derived from human Rb around the phosphorylation site of Ser807 YI(p-S)PLK |
| UniProt ID | P06400 |
| MW | 102 kDa |
| Tested applications | ICC, IF, IHC-Fr, IHC-P, WB |
| Dilution range | WB=1:500-2000, IHC-P=1:50-200, IHC-F=1:50-200, ICC/IF=1:50-200, IF=1:50-200 |
| Antibody Type | Recombinant Antibody |
| Clone Number | 2D10 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Alternative names | Rb(S807); Rb(phospho-Ser807); Rb(phospho S807); p- Read more... |
| Note | For research use only |
Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Phospho-Rb (Ser807)) Monoclonal Antibody, Unconjugated (orb559130) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
