Phospho-Raptor(S863) Antibody

SKU: orb1931134

Description

Phospho-Raptor(S863) Antibody

Research Area

Cell Biology, Signal Transduction

Images & Validation

• Tested Applications: IHC-P, WB
• Dilution Range: IHC-P - 1:100, WB - 1:500
• Reactivity: Human, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Molecular Weight: 149038 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.05% (V/V) Proclin 300. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

KIAA1303, RAPTOR

Dot blot analysis of anti-raptor-pS863 Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.

Immunohistochemical analysis on paraffin-embedded Human skeletal muscle tissue. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH9.0). Samples were incubated with primary antibody (1:100) for 1 hour at room temperature. Undiluted CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.

Western blot analysis of lysates from Hela cell line, untreated or treated with TPA, 200nM, using Phospho-Raptor (S863) Antibody (upper) or tubulin (lower).

Quick Database Links

UniProt

Q8N122