Phospho-RAF1(S338) Antibody

SKU: orb1931186

Description

Phospho-RAF1(S338) Antibody

Research Area

Cancer Biology, Cell Biology, Immunology & Inflammation, Immunology & inflammation, Metabolism Research, Signal Transduction

Images & Validation

• Tested Applications: DOT, WB
• Dilution Range: DB - 1:500, WB - 1:1000
• Reactivity: Human
• Predicted Reactivity: Bovine, Gallus, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Molecular Weight: 73052 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

RAF

Dot blot analysis of anti-RAF1-pS338 Phospho-specific Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.

Western blot analysis of Phospho-RAF1-pS338 Antibody in human TPA activated Hela cell line lysates. Phospho-RAF1 (arrow) was detected using the purified PAb. (0: without TPA; 1:60 ug/ml TPA, 15 min; 2: 60 ug/ml TPA, 30min; 3: 60 ug/ml TPA, 45min; 4: 125ug/ml TPA, 15 min; 5: 125ug/ml TPA, 30min; 6: 125ug/ml TPA, 45min; 7: 250 ug/ml TPA, 15 min; 8: 250 ug/ml TPA, 30min; 9: 250 ug/ml, 45min)

Quick Database Links

UniProt

P04049