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Phospho-RAF1 (Ser259) Recombinant Rabbit Monoclonal Antibody
Catalog Number: orb559132
| Catalog Number | orb559132 |
|---|---|
| Category | Antibodies |
| Description | Phospho-RAF1 (Ser259) Recombinant Rabbit Monoclonal Antibody |
| Target | RAF1 |
| Clonality | Recombinant |
| Species/Host | Rabbit |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Reactivity | Human |
| Predicted Reactivity | Mouse, Rat |
| Form/Appearance | Liquid |
| Concentration | 1mg/ml |
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Immunogen | KLH conjugated Synthesised phosphopeptide derived from human RAF1 around the phosphorylation site of Ser259 ST(p-S)TP |
| UniProt ID | P04049 |
| MW | 73 kDa |
| Tested applications | WB |
| Dilution range | WB=1:1000-2000 |
| Antibody Type | Recombinant Antibody |
| Clone Number | 2A1 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Alternative names | Raf1(S259); RAF1(phospho S259); Raf1(phospho-Ser25 Read more... |
| Note | For research use only |
Western blot analysis of Phospho-Raf1 (S259) on Hela cell lysates with Rabbit anti-Phospho-Raf1 (S259) antibody (orb559132) at 1/500 dilution. Lysates/proteins at 10 µg/Lane. Predicted band size: 73 kDa, Observed band size: 65 kDa, Exposure time: 1 minute, 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (orb559132) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300000 dilution was used for 1 hour at room temperature.
