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Item 1 of 4
Phospho-PTEN (Ser380) Recombinant Rabbit Monoclonal Antibody
Catalog Number: orb500055
| Catalog Number | orb500055 |
|---|---|
| Category | Antibodies |
| Description | Phospho-PTEN (Ser380) Recombinant Rabbit Monoclonal Antibody |
| Species/Host | Rabbit |
| Clonality | Recombinant |
| Clone Number | 4C9 |
| Tested applications | ICC, IF, IHC-Fr, IHC-P, WB |
| Predicted Reactivity | Mouse, Rat |
| Reactivity | Human, Mouse, Rat |
| Isotype | IgG |
| Immunogen | KLH conjugated synthesised phosphopeptide derived from human PTEN around the phosphorylation site of Ser380 RY(p-S)DT |
| Antibody Type | Recombinant Antibody |
| Concentration | 1mg/ml |
| Dilution range | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100-500, IF=1:20-200 |
| Form/Appearance | Liquid |
| Conjugation | Unconjugated |
| MW | 44 kDa |
| Target | PTEN |
| UniProt ID | P60484 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Alternative names | PTEN(phospho S380); ITGA 2; MGC11227; MHAM; MMAC 1 Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Phospho-PTEN (Ser380)) Monoclonal Antibody, Unconjugated (orb500055) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human gastric carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Phospho-PTEN (Ser380)) Monoclonal Antibody, Unconjugated (orb500055) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human lung carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Phospho-PTEN (Ser380)) Monoclonal Antibody, Unconjugated (orb500055) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Sample: Lane 1: Rat Cerebrum tissue lysates, Lane 2: Human SH-SY5Y cell lysates, Primary: Anti-Phospho-PTEN (Ser380) (orb500055) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 44 kDa, Observed band size: 55 kDa.
