Phospho-PTEN (Ser380) (NA9) rabbit mAb Antibody

SKU: orb1946173

Description

PTEN has been identified as a tumor suppressor gene and has been found to be mutated in a significant number of human cancers, including prostate, brain, and breast cancer. PTEN shares sequence homology with the protein-tyrosine phosphatase (PTPase) family of proteins and negatively regulates the PI3K/Akt pathway. PTEN de-phosphorylates target proteins, and recombinant PTEN has been shown to have phosphoinositide 3-phosphhatase and inositol phosphate 3-phosphatase activity. Studies of primary tumor cells show a loss of PTEN expression after metastasis to the brain, via astrocyte-derived microRNAs. A cluster of phosphorylation sites (S380, T382, T383, and S385) in the C-terminal tail of PTEN drive a conformational change that reduces PTEN activity by inhibiting membrane interactions.

Images & Validation

• Tested Applications: FC
• Dilution Range: 1µg/mL - 0.001µg/mL. It is recommended that the reagent be titrated for optimal performance for each application. See product image legends for additional information.
• Reactivity: Human, Mouse

Key Properties

• Host: Rabbit
• Clonality: Monoclonal
• Isotype: Rabbit IgG1k
• Clone No.: PTENS380-NA9
• Immunogen: A synthetic phospho-peptide corresponding to residues surrounding Ser380 of human phospho PTEN
• Purification: Protein A+G
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 1X PBS, 0.02% NaN3, 50% Glycerol, 0.1% rAlbumin
• Concentration: 0.5 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase, Mutated in multiple advanced cancers 1, MMAC1, Phosphatase and tensin homolog, TEP1

Flow cytometric analysis of A431 cells, untreated and unstained as negative control (blue) or untreated and stained (green) or treated with lambda phosphatase and stained (red) using Phospho-PTEN (S380) antibody, PTENS380-NA9 at 0.1 ug/mL, Cat. #orb1946173.

Peptide blocking flow cytometric analysis of A431 cells secondary antibody only negative control (light blue) or untreated (red) or treated with EGF (green) or untreated and blocked with phospho-peptide (black) or EGF and blocked with phospho peptide (gold) or untreated and blocked with non-phospho peptide (dark blue) or EGF and blocked with non-phospho peptide (purple) using Phospho-PTEN (S380) antibody PTENS380-NA9 0.05 μg/mL. Cat. #orb1946173.

PTENS380-NA9 recognizes basal phosphorylation levels in mouse cells. Flow cytometric analysis of L929 cells secondary antibody only (blue) or 0.1 μg/mL of isotype control Cat. #orb1946241 (orange) or of Phospho-PTEN (S380) antibody PTENS380-NA9 (green) Cat. #orb1946173.

Quick Database Links

UniProt

P60484