Phospho-PLCg2 (Tyr759) (G3) rabbit mAb Antibody

SKU: orb1946341

Description

The PLC-gamma isoforms of the PI-PLC family of lipases are regulated by growth factor receptors and B- and T-cell antigen receptors. While PLCg1 is expressed ubiquitously, PLCg2 is predominantly expressed in liver cells. PLCg2 plays a dominant role in B-cell signaling. Btk directly phosphorylates PLCg2, though the Syk kinase and BLNK adaptor protein are required. Both Tyr753 and Tyr759 have been identified as important phosphorylation sites for PLCg2 activation in B-cells. PLCg2 missense mutations and genomic deletions have been identified autoinflammatory diseases in humans. These include gain-of-function mutations, such as S707T, that possibly introduce an additional phosphorylation site and increase basal PLCg2 activity.

Images & Validation

• Tested Applications: FC, WB
• Dilution Range: 1µg/mL - 0.001µg/mL. It is recommended that the reagent be titrated for optimal performance for each application. See product image legends for additional information.
• Reactivity: Human, Mouse

Key Properties

• Host: Rabbit
• Clonality: Monoclonal
• Isotype: Rabbit IgG1k
• Clone No.: PLCG2Y759-G3
• Immunogen: A synthetic phospho-peptide corresponding to residues surrounding Tyr759 of human phospho PLCg2.
• Purification: Protein A+G
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 1X PBS, 0.02% NaN3, 50% Glycerol, 0.1% rAlbumin
• Concentration: 0.5 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-2, Phosphoinositide phospholipase C-gamma-2, PLC-IV, PLC-gamma-2, PLCG2

Western blot analysis of Ramos cell extract untreated or treated with pervanadate using Phospho-PLCγ2 (Tyr759) antibody PLCG2Y759-G3. Cat. #orb1946341.

Flow cytometric analysis of NIH3T3 cells secondary antibody only negative control (blue) or 0.1 μg/mL of isotype control Cat. #orb1946241 (orange) or treated with imatinib (red) or with pervanadate (green) using Phospho-PLCγ2 (Tyr759) antibody PLCG2Y759-G3 at 0.1 μg/mL. Cat #orb1946341.

Flow cytometric analysis of Ramos cells secondary antibody only negative control (blue) or untreated (red) or treated with pervanadate (green) using 0.01 ug/mL Phospho-PLCγ2 (Tyr759) antibody PLCG2Y759-G3. Cat. #orb1946341.

Western blot analysis of Ramos cell extract untreated or treated with pervanadate using 0.01 μg/mL Phospho-PLCγ2 (Tyr759) antibody PLCg2Y759-G3 Cat. #orb1946341 or Company C antibody at 0.06 μg/mL (manufacturer's recommended concentration) developed using the same exposure.

Quick Database Links

UniProt

P16885