Phospho-PKCa (Thr497) (F1) rabbit mAb Antibody

• Catalog Number: orb1946091
• Category: Antibodies
• Description: Phospho-PKCa (Thr497) (F1) rabbit mAb Antibody
• Clonality: Monoclonal
• Clone Number: PKCaT497-F1
• Tested applications: FC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgGk
• Immunogen: A synthetic phospho-peptide corresponding to residues surrounding Thr497 of human phospho PKCα
• Concentration: 0.5 mg/ml
• Dilution range: 1µg/mL – 0.001µg/mL. It is recommended that the reagent be titrated for optimal performance for each application. See product image legends for additional information.
• Conjugation: Unconjugated
• UniProt ID: P17252
• Storage: -20°C
• Buffer/Preservatives: 1X PBS, 0.02% NaN3, 50% Glycerol, 0.1% rAlbumin
• Alternative names: Protein kinase C alpha type, PKC-alpha, PRKCA, PKC
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Flow cytometric analysis of K562 cells secondary antibody only negative control (blue) or untreated (red) or treated with EGF + pervanadate (green) using PKCα (T497) antibody PKCaT497-F1 at 0.1 μg/mL. Cat. #orb1946091.

Western blot analysis of HT1080 cell extract, untreated or treated with TPA using 0.05 ug/mL Phospho-PKCa (Thr497) antibody AWBPKCAT497-F1. Cat. #orb1946091

Western blot analysis of HT1080 cell extract untreated or treated with TPA using 0.05 μg/mL Phospho-PKCα (Thr497) antibody PKCaT497-F1 Cat. #orb1946091 or Company A antibody at 1 μg/mL (manufacturer's recommended concentration) developed using the same exposure.

Flow cytometric analysis of 3T3 cells, secondary antibody only negative control (blue) or treated with imatinib (grey) or with pervanadate (orange) using 0.1 μg/mL isotype control Cat. #orb1946241, or imatinib (red) or pervanadate (green) using PKCα (T497) antibody PKCaT497-F1 at 0.1μg/mL Cat. #orb1946091.

Western blot analysis of C6 cell extract treated with Anisomycin using 0.1 μg/mL PKCα (T497) antibody PKCaT497-F1. Cat. #orb1946091.