Phospho-PKC (pan) (gamma Thr514) (PF4) rabbit mAb

• Catalog Number: orb1946008
• Category: Antibodies
• Description: Phospho-PKC (pan) (gamma Thr514) (PF4) rabbit mAb
• Clonality: Monoclonal
• Clone Number: PKCgT514-PF4
• Tested applications: FC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgGk
• Immunogen: A synthetic phospho-peptide corresponding to residues surrounding Thr514 of human phospho PKCγ
• Concentration: 0.5 mg/ml
• Dilution range: 1µg/mL – 0.001µg/mL. It is recommended that the reagent be titrated for optimal performance for each application. See product image legends for additional information.
• Conjugation: Unconjugated
• UniProt ID: Q02156, Q04759, P24723, P05771, P05129, Q05655, P17252
• Storage: -20°C
• Buffer/Preservatives: 1X PBS, 0.02% NaN3, 50% Glycerol, 0.1% rAlbumin
• Alternative names: Protein kinase C gamma type, PRKCG, PKCG
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Flow cytometric analysis of HT1080 cells treated with staurosporine (red) or untreated (green) using Phospho-PKCg (Thr514) (PF4) Rabbit mAb. at 0.05 ug/mL, PKCgT514-PF4 #orb1946008, or concentration-matched Rabbit (G9) mAb IgG Isotype Control #orb1946241 for cells treated with staurosporine (black) or untreated (blue). Flow cytometric analysis of HT1080 cells, treated with staurosporine and stained with the secondary antibody only as negative control (blue) or treated with staurosporine (red) or untreated (green) using Phospho-PKC (pan) (gamma Thr514) antibody PKCgT514-PF4 at 0.05 μg/mL. Cat. #orb1946008.

Flow cytometric analysis of Jurkat cells, treated with K252a and stained with the secondary antibody only negative control (blue) or treated with K252a (red) or untreated (green) using Phospho-PKC (pan) (gamma Thr514) antibody PKCgT514-PF4 at 0.1 μg/mL. Cat. #orb1946008.

Peptide blocking flow cytometric analysis of HT1080 cells secondary antibody only negative control (light blue) or untreated (red) or treated with staurosporine (green) or untreated and blocked with phospho-peptide (black) or treated with staurosporine and blocked with phospho peptide (gold) or untreated and blocked with non-phospho peptide (dark blue) or treated with staurosporine and blocked with non-phospho peptide (purple) using Phospho-PKCg (Thr514) antibody PKCGT514-PF4 at 0.05 μg/mL. Cat. #orb1946008.

Western blot analysis of NIH3T3 cell extract untreated or treated with TPA using Phospho-PKC (pan) (gamma Thr514) antibody PKCgT514-PF4 at 0.05 ug/mL. Cat. #orb1946008.

Western blot analysis of C6 cell extract untreated or treated with staurosporine using Phospho-PKC (pan) gamma (Thr514) antibody PKCgT514-PF4 at 0.1 ug/mL. Cat. #orb1946008.