Phospho-PI3 Kinase p85 (Tyr458)/p55 (Tyr199) (1A11) rabbit mAb

• Catalog Number: orb1946298
• Category: Antibodies
• Description: Phospho-PI3 Kinase p85 (Tyr458)/p55 (Tyr199) (1A11) rabbit mAb
• Clonality: Monoclonal
• Isotype: Rabbit IgGk
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Concentration: 0.5 mg/ml
• Buffer/Preservatives: 1X PBS, 0.02% NaN3, 50% Glycerol, 0.1% rAlbumin
• Purification: Protein A+G
• Immunogen: A synthetic phospho-peptide corresponding to residues surrounding Tyr458 of human phospho PI3K p85
• UniProt ID: P27986, Q92569, O00459
• Tested applications: FC, WB
• Dilution range: 1µg/mL – 0.001µg/mL. It is recommended that the reagent be titrated for optimal performance for each application. See product image legends for additional information.
• Cross Reactivity: Predicted to work with mouse, rat, and other homologues.
• Clone Number: PI3KY458-1A11
• Storage: -20°C
• Alternative names: Phosphatidylinositol 3-kinase regulatory subunit a
• Note: For research use only

Western blot analysis of NIH3T3 cell extract, untreated or treated with UV using 0.5 ug/mL Phospho-PI3Kinase p85 (Tyr458) /p55 (Tyr199) antibody PI3KY458-1A11. Cat #orb1946298.

Flow cytometric analysis of NIH3T3 cells secondary antibody only negative control (blue) or 0.1 μg/mL of isotype control Cat. #orb1946241 (orange) or treated with imatinib (red) or with pervanadate (green) using Phospho-PI3 Kinase p85 (Tyr458) /p55 (Tyr199) antibody PI3KY458-1A11 at 0.1 μg/mL. Cat #orb1946298.

Peptide blocking flow cytometric analysis of NIH3T3 cells secondary antibody only negative control (light blue) or treated with imatinib (red) or with pervanadate (green) or imatinib and blocked with phospho-peptide (black) or pervanadate and blocked with phospho peptide (gold) or imatinib and blocked with non-phospho peptide (dark blue) or pervanadate and blocked with non-phospho peptide (purple) using Phospho-PI3 Kinase p85 (Tyr458) /p55 (Tyr199) antibody PI3KY458-1A11 at 0.1 μg/mL. Cat #orb1946298.

Flow cytometric analysis of Ramos cells secondary antibody only negative control (blue) or untreated (red) or treated with pervanadate (green) using Phospho-PI3 Kinase p85 (Tyr458) /p55 (Tyr199) antibody PI3KY458-1A11. Cat.#orb1946298.