Phospho-PDK1 (Ser241) (F7) rabbit mAb Antibody

SKU: orb1946124

Description

PDK1 is a Ser/Thr kinase that is ubiquitously expressed throughout human tissues. PDK1 phosphorylates protein kinase B (PKB or Akt) at both Thr308 and Ser473 in vivo. PDK1 is active and phosphorylated in basal conditions, where it exists predominantly in the cytosol with a small fraction at the plasma membrane. This membrane association is likely mediated by PDK1 binding to phospholipids through its PH domain, which has been found to have strong affinity to specific phospholipids. PDK1 is a master regulator of at least 23 related protein kinases, and more than 50% of all human cancers show significant overstimulation of the PDK1 pathway. Most small-molecule inhibitors of PDK1 target the ATP binding site.

Images & Validation

• Tested Applications: FC
• Dilution range: 1µg/mL - 0.001µg/mL. It is recommended that the reagent be titrated for optimal performance for each application. See product image legends for additional information.
• Reactivity: Human

Key Properties

• Host: Rabbit
• Clonality: Monoclonal
• Isotype: Rabbit IgG1k
• Clone No.: PDK1S241-F7
• Immunogen: A synthetic phospho-peptide corresponding to residues surrounding Ser241 of human phospho PDK1
• Purification: Protein A+G
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 1X PBS, 0.02% NaN3, 50% Glycerol, 0.1% rAlbumin
• Concentration: 0.5 mg/mL
• Disclaimer: For research use only

Alternative Names

3-phosphoinositide-dependent protein kinase 1, PDPK1

Peptide blocking flow cytometric analysis of 293T cells secondary antibody only negative control (light blue) or treated with K252 (red) or with pervanadate (green) or K252 and blocked with phospho-peptide (black) or pervanadate and blocked with phospho peptide (gold) or K252 and blocked with non-phospho peptide (dark blue) or pervanadate and blocked with non-phospho peptide (purple) using Phospho-NDRG1 (Thr346) antibody PDPK1S241-F7 at 0.1 μg/mL. Cat. #orb1946124.

Flow cytometric analysis of 293T cells secondary antibody only negative control (blue) or treated with K252 (red) or with pervanadate (green) using Phospho-PDPK1 (Ser241) antibody PDPK1S241-F7 at 0.1 μg/mL. Cat. #orb1946124.

Quick Database Links

UniProt

O15530