Phospho-PAK1/2/3 (Ser144/Ser141/Ser154) Rabbit mAb

• Catalog Number: orb1564478
• Category: Antibodies
• Description: Phospho-PAK1/2/3 (Ser144/Ser141/Ser154) Rabbit mAb
• Clonality: Monoclonal
• Clone Number: R04-2A4
• Tested applications: ICC, IF, IHC-Fr, IHC-P, IP, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: A synthetic phosphopeptide corresponding to residues surrounding Ser144 of human PAK1
• Antibody Type: Primary Antibody
• Concentration: 0.3 mg/ml
• Dilution range: WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200 IP: 1/20
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Calculated MW: 62 kDa; Observed MW: 62 kDa
• Target: PAK3
• UniProt ID: O75914
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% rAlbumin.
• Alternative names: PAK3; OPHN3; Serine/threonine-protein kinase PAK 3
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of PAK1 (Phospho-Ser144) /PAK2 (Phospho-Ser141) /PAK3 (Phospho-Ser154) in C6, 3T3, Hela lysates using Phospho-PAK1/2/3 (Ser144/Ser141/Ser154) antibody.

Immunocytochemistry analysis of PAK1 (Phospho-Ser144) /PAK2 (Phospho-Ser141) /PAK3 (Phospho-Ser154) (green) in Hela using PAK1 (Phospho-Ser144) /PAK2 (Phospho-Ser141) /PAK3 (Phospho-Ser154) antibody, and DAPI (blue).

Immunohistochemistry analysis of paraffin-embedded Human breast cancer using Phospho-PAK1 (Ser144) /2 (Ser141) /3 (Ser154) antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.