Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (A11) rabbit mAb

• Catalog Number: orb1946370
• Category: Antibodies
• Description: Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (A11) rabbit mAb
• Clonality: Monoclonal
• Clone Number: ERK12T202Y204-A11
• Tested applications: FC, WB
• Reactivity: Human, Mouse
• Isotype: Rabbit IgGk
• Immunogen: A synthetic phospho-peptide corresponding to residues surrounding Thr202/Tyr204 of human phospho Erk1/2.
• Concentration: 0.5 mg/ml
• Dilution range: 1µg/mL – 0.001µg/mL. It is recommended that the reagent be titrated for optimal performance for each application. See product image legends for additional information.
• Conjugation: Unconjugated
• UniProt ID: P28482, P27361
• Storage: -20°C
• Buffer/Preservatives: 1X PBS, 0.02% NaN3, 50% Glycerol, 0.1% rAlbumin
• Alternative names: Mitogen-activated protein kinase 3, MAPK3, ERK2, p
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Flow cytomeric analysis of jurket cells treated with U0126 (red) or TPA (green) and stained using Phospho-ERK1/2 (Thr202/Tyr204) antibody ERK12T202Y204-A11. Cat. #orb1946370 or concentration match isotope control Cat#orb1946241 for cells treated with U0126 (gray) or treated with TPA (blue).

ERK12T202Y204-A11 recognizes basal phosphorylation levels in mouse cells. Flow cytometric analysis of L929 cells secondary antibody only (blue) or 0.1 μg/mL of isotype control Cat. #orb1946241 (orange) or of Phospho-ERK1/2 (Thr202/Tyr204) antibody ERK12T202Y204-A11 (green) Cat. #orb1946370.

Western blot analysis of 293T cell extract treated with U0126 or TPA using Phospho-ERK1/2 (Thr202/Tyr204) antibody ERK12T202Y204-A11 at 0.1 μg/mL. Cat. #orb1946370.

Western blot analysis of Ramos cell extract untreated or treated with U0126 followed by no treatment or treatment with PMA using Phospho-ERK1/2 (Thr202/Tyr204) antibody ERK12T202Y204-A11. Cat. #orb1946370.

Flow cytometric analysis of Jurkat cells, secondary antibody only negative control (blue), or treated with U0126 (red) or with PMA (green) using Phospho-Erk1/2 (T202/Y204) antibody ERK12T202Y204-A11 (Cat. #orb1946370) or Company C antibody at 0.43 μg/mL (manufacturer's recommended concentration).

Western blot analysis of Ramos cells treated with U0126 or treated with TPA using 0.05 ug/mL of Phospho ERK1/2 (Thr202/Tyr204) antibody ERK12T202Y204-A11 Cat. #orb1946370 or Company C antibody at recommended concentration of 0.34 ug/mL.