Phospho-NDRG1 (Thr346) (F5) rabbit mAb Antibody

• Catalog Number: orb1946264
• Category: Antibodies
• Description: Phospho-NDRG1 (Thr346) (F5) rabbit mAb Antibody
• Clonality: Monoclonal
• Clone Number: NDRG1T346-F5
• Tested applications: FC, WB
• Reactivity: Human, Mouse
• Isotype: Rabbit IgGk
• Immunogen: A synthetic phospho-peptide corresponding to residues surrounding Thr346 of human phospho NDRG1
• Concentration: 0.5 mg/ml
• Dilution range: 1µg/mL – 0.001µg/mL. It is recommended that the reagent be titrated for optimal performance for each application. See product image legends for additional information.
• Conjugation: Unconjugated
• UniProt ID: Q92597
• Storage: -20°C
• Buffer/Preservatives: 1X PBS, 0.02% NaN3, 50% Glycerol, 0.1% rAlbumin
• Alternative names: Differentiation-related gene 1 protein, N-myc down
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Peptide blocking flow cytometric analysis of THP1 cells secondary antibody only negative control (light blue) or untreated (orange) using isotype control antibody #orb1946241 or untreated (red) or with IFNα + IL-4 + pervanadate (green) or untreated and blocked with phospho-peptide (black) or treated and blocked with phospho peptide (gold) or untreated and blocked with non-phospho peptide (dark blue) or treated and blocked with non-phospho peptide (purple) using Phospho-NDRG1 (Thr346) antibody NDRG1T346-F5 at 0.05 μg/mL. Cat. #orb1946264.

Flow cytometric analysis of THP1 cells secondary antibody only negative control (blue) or untreated (red) or treated with IFNα + IL-4 + pervanadate (green) using Phospho-NDRG1 (Thr346) antibody NDRG1T346-F5 at 0.05 μg/mL. Cat. #orb1946264.

Western blot analysis of C2C12 cell extract untreated or treated with LY294002 using 0.05 ug/mL Phospho-NDRG1 (Thr346) antibody NDRG1T346-F5 Cat. #orb1946264.

Western blot analysis of C2C12 cell extract untreated or treated with LY294002 using 0.05 μg/mL Phospho-NDRG1 (Thr346) antibody NDRG1T346-F5 Cat. #orb1946264 or Company C antibody at 0.07 μg/mL (manufacturer's recommended concentration) developed using the same exposure.