Phospho-Nanog(S71) Antibody

SKU: orb1931082

Description

Phospho-Nanog(S71) Antibody

Research Area

Cancer Biology, Signal Transduction, Stem Cell & Developmental Biology

Images & Validation

• Tested Applications: DOT, IF
• Dilution Range: IF - 1:10-50, DB - 1:500
• Reactivity: Human
• Predicted Reactivity: Monkey

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Molecular Weight: 34620 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Dot blot analysis of anti-Phospho-Nanog-S71 Antibody on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.

Fluorescent confocal image of SY5Y cells stained with Phospho-Nanog-S71 antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min), then incubated with Phospho-Nanog-S71 primary antibody (1:500, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1 h). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (5.25 μM, 25 min). Phospho-Nanog immunoreactivity is localized very specifically to the nuclei of the SY5Y cells.

Quick Database Links

UniProt

Q9H9S0