Phospho-MYT1(T495) Antibody

SKU: orb34472

Description

Peptide affinity purified rabbit polyclonal antibody.

Research Area

Cell Biology, Signal Transduction

Images & Validation

• Tested Applications: DOT, IHC-P, WB
• Dilution Range: WB - 1:1000, IHC-P - 1:50-100, DB - 1:500
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: RB7898
• Immunogen: Synthetic Peptide
• Target: PKMYT1
• Molecular Weight: 54521
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Anti-Membrane-associated tyrosine-and threonine-specific cdc2-inhibitory kinase antibody, anti-Myt1 kinase antibody, anti-PKMYT1 antibody, anti-MYT1 antibody

The anti-Phospho-MYT1-T495 Antibody is used in Western blot to detect Phospho-MYT1-T495 in HL60 (left) and SK-BR-3 (right) tissue lysates.

Dot blot analysis of anti-Phospho-MYT1-T495 Antibody on nitrocellulose membrane. 50 ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Quick Database Links

Gene Symbol

PKMYT1

UniProt

Q99640

RefSeq (Protein):NP_872629.1, NP_001245379.1, NP_004194.3, NP_001245380.1