Phospho-Mst1(Thr183) + Mst2(Thr180) Rabbit pAb, FITC conjugated Discontinued

SKU: orb9297

Description

Rabbit polyclonal to Mst1 (phospho-Thr183) antibody conjugated to FITC

Research Area

Apoptosis, Apoptotic, Cancer, Cell Biology, Epigenetics, Immunology, Neuroscience, Signaling Pathways

Images & Validation

• Tested Applications: IF
• Dilution Range: IF=1:100-500
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Equine, Gallus, Mouse, Rabbit, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from human Mst1 around the phosphorylation site of Thr183
• Target: STK4
• Molecular Weight: 19/37/56kDa
• Purification: Affinity purified by Protein A
• Conjugation: FITC

Storage & Handling

• Storage: Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
• Form/Appearance: Lyophilized or Liquid
• Buffer/Preservatives: 0.01M TBS(pH7.4) with 1% rAlbumin, 0.03% Proclin300 and 50% Glycerol.
• Concentration: - 100 μg (in 100 μl): 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti-Mst1 (phospho-Thr183) antibody, anti-STK4 antibody, anti-Krs2 antibody, anti-Mammalian STE20 like protein kinase 1 antibody, anti-Mammalian sterile 20 like 1 antibody, anti-Serine/threonine protein kinase Krs 2 antibody, anti-STE20 like kinase MST1 antibody, anti-STK4(Thr183)/STK3(Thr180) antibody, anti-Mst1/2(Thr183/Thr180) antibody, anti-STK4/3(Thr183/Thr180) antibody

Blank control (Black line): HeLa. Primary Antibody (green line): Rabbit Phospho-Mst1 (Thr183) + Mst2 (Thr180) antibody, dilution: 3 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG, dilution: 3 µg/Test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20000 events was performed.

Blank control: Mouse spleen. Primary Antibody (green line): Rabbit Anti-Phospho-Mst1 (Thr183) + Mst2 (Thr180) antibody (orb9297), dilution: 2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20000 events was performed.

Quick Database Links

Gene Symbol

STK4

UniProt

Q13043

Moon, Sohyeon et al. STK3/4 Expression Is Regulated in Uterine Endometrial Cells during the Estrous Cycle Cells, 8, (2019)

PubMed: 31847471