Phospho-MSK1 (Thr581) rabbit mAb Antibody

SKU: orb1946223

Description

MSK1 (mitogen and stress activated protein kinase 1, phospho MSK1) is activated by Erk in response to growth factors and by and p38 in response to cellular stress (1). MSK1 is similar to RSK1 in that it has two kinase domains and a connecting regulatory linker region (2). S364/S381 phosphorylation activates RSK1 (3), which is analogous to residues S360 and S376 of MSK1, which may be important for phospho MSK1 activity.

Research Area

Signal Transduction

Images & Validation

• Tested Applications: FC, WB
• Dilution Range: 1µg/mL - 0.001µg/mL. It is recommended that the reagent be titrated for optimal performance for each application. See product image legends for additional information.
• Reactivity: Human, Mouse, Rat

Key Properties

• Host: Rabbit
• Clonality: Monoclonal
• Isotype: Rabbit IgG1k
• Clone No.: BC7
• Immunogen: A synthetic phospho-peptide corresponding to residues surrounding Thr581 of human phospho MSK1
• Purification: Protein A+G
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 1X PBS, 0.02% NaN3, 50% Glycerol, 0.1% rAlbumin
• Concentration: 0.5 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Ribosomal protein S6 kinase alpha-5, S6K-alpha-5, 90 kDa ribosomal protein S6 kinase 5, Nuclear mitogen- and stress-activated protein kinase 1, RSK-like protein kinase, RSKL, RPS6KA5

Western blot analysis of 293T cell extract untreated or treated with UV using 0.1 ug/mL Phospho-MSK1 (Thr581) antibody MSK1T581-A5. Cat. #orb1946223.

Flow cytometric analysis of U937 cells secondary antibody only negative control (blue) or untreated (red) or treated with CalA (green) using Phospho-MSK1 (T581) antibody MSK1T581-A5 1.0 μg/mL. Cat. #orb1946223.

Peptide blocking flow cytometric analysis of C6 cells secondary antibody only negative control (light blue) or pervanadate-treated and stained using 0.1μg/mL isotype control Cat. #orb1946241 (orange) or pervanadate-treated and stained using 0.1μg/mL Phospho-MSK1 (Thr581) antibody MSK1T581-A5 Cat. #orb1946223 (green) or pervanadate and blocked with phospho peptide (gold) or pervanadate and blocked with non-phospho peptide (purple).

Flow cytometric analysis of C2C12 cells secondary antibody only negative control (blue) or 0.1 μg/mL of isotype control Cat. #orb1946241 (orange) or treated with imatinib (red) or with pervanadate (green) using Phospho-MSK1 (Thr581) antibody MSK1T581-A5 at 0.1 μg/mL. Cat #orb1946223.

Peptide blocking flow cytometric analysis of K562 cells secondary antibody only negative control (light blue) or imatinib-treated (red) or pervanadate-treated (green) or imatinib and blocked with phospho-peptide (black) or pervanadate and blocked with phospho peptide (gold) or imatinib and blocked with non-phospho peptide (dark blue) or pervanadate and blocked with non-phospho peptide (purple) Phospho-MSK1 (Thr581) antibody MSK1T581-A5 at 0.1μg/mL. Cat. #orb1946223.

Western blot analysis of 293T cell extract untreated or treated with staurosporine using 0.1 μg/mL Phospho-MSK1 (Thr581) antibody MSK1T581-A5 Cat. #orb1946223 or Company C antibody at 0.02 μg/mL (manufacturer's recommended concentration) developed using the same exposure.

Quick Database Links

UniProt

O75582