Phospho-MSK1 (Thr581) (A5) rabbit mAb

• Catalog Number: orb1946223
• Category: Antibodies
• Description: Phospho-MSK1 (Thr581) (A5) rabbit mAb
• Clonality: Monoclonal
• Clone Number: MSK1T581-A5
• Tested applications: FC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgGk
• Immunogen: A synthetic phospho-peptide corresponding to residues surrounding Thr581 of human phospho MSK1
• Concentration: 0.5 mg/ml
• Dilution range: 1µg/mL – 0.001µg/mL. It is recommended that the reagent be titrated for optimal performance for each application. See product image legends for additional information.
• Conjugation: Unconjugated
• UniProt ID: O75582
• Storage: -20°C
• Buffer/Preservatives: 1X PBS, 0.02% NaN3, 50% Glycerol, 0.1% rAlbumin
• Alternative names: Ribosomal protein S6 kinase alpha-5, S6K-alpha-5,
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of 293T cell extract untreated or treated with UV using 0.1 ug/mL Phospho-MSK1 (Thr581) antibody MSK1T581-A5. Cat. #orb1946223.

Flow cytometric analysis of U937 cells secondary antibody only negative control (blue) or untreated (red) or treated with CalA (green) using Phospho-MSK1 (T581) antibody MSK1T581-A5 1.0 μg/mL. Cat. #orb1946223.

Peptide blocking flow cytometric analysis of C6 cells secondary antibody only negative control (light blue) or pervanadate-treated and stained using 0.1μg/mL isotype control Cat. #orb1946241 (orange) or pervanadate-treated and stained using 0.1μg/mL Phospho-MSK1 (Thr581) antibody MSK1T581-A5 Cat. #orb1946223 (green) or pervanadate and blocked with phospho peptide (gold) or pervanadate and blocked with non-phospho peptide (purple).

Flow cytometric analysis of C2C12 cells secondary antibody only negative control (blue) or 0.1 μg/mL of isotype control Cat. #orb1946241 (orange) or treated with imatinib (red) or with pervanadate (green) using Phospho-MSK1 (Thr581) antibody MSK1T581-A5 at 0.1 μg/mL. Cat #orb1946223.

Peptide blocking flow cytometric analysis of K562 cells secondary antibody only negative control (light blue) or imatinib-treated (red) or pervanadate-treated (green) or imatinib and blocked with phospho-peptide (black) or pervanadate and blocked with phospho peptide (gold) or imatinib and blocked with non-phospho peptide (dark blue) or pervanadate and blocked with non-phospho peptide (purple) Phospho-MSK1 (Thr581) antibody MSK1T581-A5 at 0.1μg/mL. Cat. #orb1946223.

Western blot analysis of 293T cell extract untreated or treated with staurosporine using 0.1 μg/mL Phospho-MSK1 (Thr581) antibody MSK1T581-A5 Cat. #orb1946223 or Company C antibody at 0.02 μg/mL (manufacturer's recommended concentration) developed using the same exposure.