Phospho-MKK3 (Ser189)/MKK6 (Ser207) (D3) rabbit mAb Antibody

• Catalog Number: orb1946168
• Category: Antibodies
• Description: Phospho-MKK3 (Ser189)/MKK6 (Ser207) (D3) rabbit mAb Antibody
• Clonality: Monoclonal
• Clone Number: MKK3S189MKK6S207-D3
• Tested applications: FC, WB
• Reactivity: Human, Mouse
• Isotype: Rabbit IgGk
• Immunogen: A synthetic phospho-peptide corresponding to residues surrounding Ser189 of human phospho MKK3 and Ser207 of human phospho MKK6.
• Concentration: 0.5 mg/ml
• Dilution range: 1µg/mL – 0.001µg/mL. It is recommended that the reagent be titrated for optimal performance for each application. See product image legends for additional information.
• Conjugation: Unconjugated
• UniProt ID: P46734
• Storage: -20°C
• Buffer/Preservatives: 1X PBS, 0.02% NaN3, 50% Glycerol, 0.1% rAlbumin
• Alternative names: Dual specificity mitogen-activated protein kinase
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of COS7 cell extract untreated or treated with UV using 0.05 μg/mL Phospho-MKK3 (Ser189) MKK6 (S207) antibody MKK3S189MKK6S207-D3. Cat. #orb1946168.

Peptide blocking flow cytometric analysis of HEK293T cells secondary antibody only negative control (light blue) or treated with K252a (red) or UV/TPA-treated (green) or K252a and blocked with phospho-peptide (black) or UV/TPA and blocked with phospho peptide (gold) or K252a and blocked with non-phospho peptide (dark blue) or UV/TPA and blocked with non-phospho peptide (purple) using Phospho-MKK3 (S189) /MKK6 (S207) antibody MKK3S189MKK6S207-D3 0.1μg/mL. Cat. # orb1946168.

MKK3S189MKK6S207-D3 recognizes basal phosphorylation levels in mouse cells. Flow cytometric analysis of 3T3 cells secondary antibody only (blue) or 0.1 μg/mL of isotype control Cat. #orb1946241 (orange) or of MKK3 (S189) /MKK6 (S207) antibody MKK3S189MKK6S207-D3 (green) Cat. #orb1946168.

Flow cytometric analysis of HEK293T cells secondary antibody only negative control (blue) or treated with K252a (red) or treated with UV + TPA (green) using 0.5 μg/mL of Phospho-MKK3 (Ser189) /MKK6 (Ser207) antibody MKK3S189MKK6S207-D3 Cat. #orb1946168.