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Item 1 of 4
Phospho-MKK3 (Ser189)/MKK6 (Ser207) (D3) rabbit mAb
Catalog Number: orb1946168
| Catalog Number | orb1946168 |
|---|---|
| Category | Antibodies |
| Description | Phospho-MKK3 (Ser189)/MKK6 (Ser207) (D3) rabbit mAb |
| Clonality | Monoclonal |
| Clone Number | MKK3S189MKK6S207-D3 |
| Tested applications | FC, WB |
| Reactivity | Human, Mouse |
| Isotype | Rabbit IgGk |
| Immunogen | A synthetic phospho-peptide corresponding to residues surrounding Ser189 of human phospho MKK3 and Ser207 of human phospho MKK6. |
| Concentration | 0.5 mg/ml |
| Dilution range | 1µg/mL – 0.001µg/mL. It is recommended that the reagent be titrated for optimal performance for each application. See product image legends for additional information. |
| Conjugation | Unconjugated |
| UniProt ID | P46734 |
| Storage | -20°C |
| Buffer/Preservatives | 1X PBS, 0.02% NaN3, 50% Glycerol, 0.1% rAlbumin |
| Alternative names | Dual specificity mitogen-activated protein kinase Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
Western blot analysis of COS7 cell extract untreated or treated with UV using 0.05 μg/mL Phospho-MKK3 (Ser189) MKK6 (S207) antibody MKK3S189MKK6S207-D3. Cat. #orb1946168.
Peptide blocking flow cytometric analysis of HEK293T cells secondary antibody only negative control (light blue) or treated with K252a (red) or UV/TPA-treated (green) or K252a and blocked with phospho-peptide (black) or UV/TPA and blocked with phospho peptide (gold) or K252a and blocked with non-phospho peptide (dark blue) or UV/TPA and blocked with non-phospho peptide (purple) using Phospho-MKK3 (S189) /MKK6 (S207) antibody MKK3S189MKK6S207-D3 0.1μg/mL. Cat. # orb1946168.
MKK3S189MKK6S207-D3 recognizes basal phosphorylation levels in mouse cells. Flow cytometric analysis of 3T3 cells secondary antibody only (blue) or 0.1 μg/mL of isotype control Cat. #orb1946241 (orange) or of MKK3 (S189) /MKK6 (S207) antibody MKK3S189MKK6S207-D3 (green) Cat. #orb1946168.
Flow cytometric analysis of HEK293T cells secondary antibody only negative control (blue) or treated with K252a (red) or treated with UV + TPA (green) using 0.5 μg/mL of Phospho-MKK3 (Ser189) /MKK6 (Ser207) antibody MKK3S189MKK6S207-D3 Cat. #orb1946168.
