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Phospho-MCM2 (Ser27) Rabbit mAb
Description
Images & Validation
−| Tested Applications | ICC, IF, IHC-Fr, IHC-P, IP, WB |
|---|---|
| Dilution range | WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200 IP: 1/20 |
| Reactivity | Human |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Clonality | Monoclonal |
| Isotype | IgG |
| Clone No. | R08-4D8 |
| Immunogen | A synthetic phosphopeptide corresponding to residues surrounding Ser27 of human MCM2 |
| Target | MCM2 |
| Molecular Weight | Calculated MW: 102 kDa; Observed MW: 125 kDa |
| Purification | Affinity Purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% rAlbumin. |
| Concentration | 0.3 mg/ml |
| Disclaimer | For research use only |
Alternative Names
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Immunocytochemistry analysis of Phospho-MCM2 (Ser27) (green) in Hela using Phospho-MCM2 (Ser27) antibody, and DAPI (blue).

Western blot analysis of MCM2 (Phospho-Ser27) in Hela, A549, HL-60, U2OS lysates using MCM2 (Phospho-Ser27) antibody.

Immunohistochemistry analysis of paraffin-embedded Human tonsil using MCM2 (Phospho-Ser27) antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
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Protocol Information
Phospho-MCM2 (Ser27) Rabbit mAb (orb1563996)
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