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Catalog Number | orb1946023 |
---|---|
Category | Antibodies |
Description | Phospho-MCM2 (Ser139) (B12) rabbit mAb Antibody |
Clonality | Monoclonal |
Clone Number | MCM2S139-B12 |
Tested applications | FC, WB |
Reactivity | Human, Mouse, Rat |
Isotype | Rabbit IgGk |
Immunogen | A synthetic phospho-peptide corresponding to residues surrounding Ser139 of human phospho MCM2 |
Concentration | 0.5 mg/ml |
Dilution range | 1µg/mL – 0.001µg/mL. It is recommended that the reagent be titrated for optimal performance for each application. See product image legends for additional information. |
Conjugation | Unconjugated |
UniProt ID | P49736 |
Storage | -20°C |
Buffer/Preservatives | 1X PBS, 0.02% NaN3, 50% Glycerol, 0.1% rAlbumin |
Alternative names | DNA replication licensing factor MCM2, Minichromos Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Flow cytometric analysis of C6 cells, secondary antibody only negative control (blue) or untreated (red) or treated with staurosporine (green) using Phospho-MCM2 (Ser139) antibody MCM2S139-B12 at 0.01 μg/mL. Cat. #orb1946023.
Western blot analysis of L929 cell extract untreated or treated with 25uM etoposide using Phospho-MCM2 (Ser139) antibody MCM2S139-B12 at 0.05 ug/mL. Cat. #orb1946023.
Peptide blocking flow cytometric analysis of C6 cells secondary antibody only negative control (light blue) or untreated (red) or treated with staurosporine (green) or untreated and blocked with phospho-peptide (black) or treated and blocked with phospho peptide (gold) or untreated and blocked with non-phospho peptide (dark blue) or treated and blocked with non-phospho peptide (purple) using Phospho-MCM2 (Ser139) antibody MCM2S139-B12 at 0.1 μg/mL. Cat. #orb1946023.
Western blot analysis of L929 cell extract untreated or treated with etoposide using 0.05 ug/mL Phospho-MCM2 (Ser139) antibody MCM2S139-B12. Cat.#orb1946023 or Company C antibody at 0.07 ug/mL (manufacturer's recommended concentration) developed using the same exposure.
Flow cytometric analysis of A431 cells, secondary antibody only negative control (blue), or untreated (grey) or treated with staurosporine (orange) using 0.01 ug/mL isotype control Cat. #orb1946241, or untreated (red) or treated (green) using Phospho-MCM2 (Ser139) antibody MCM2S139-B12 at 0.01 μg/mL. Cat. #orb1946023.
FC | |
Human, Mouse, Rat | |
Monoclonal | |
APC |
FC | |
Human, Mouse, Rat | |
Monoclonal | |
FITC |
FC | |
Human, Mouse, Rat | |
Monoclonal | |
PE |
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