Phospho-MCM2 (Ser139) (B12) rabbit mAb Antibody

SKU: orb1946023

Description

The members of minichromosome maintenance (McM) protein family 2-7 were originally identified as a group of proteins essential for DNA replication (chromosomal maintenance (1,2). They share common sequence homology to each other in their nulceotide-binding domains and are distinct subgroup of the large AAA ATPase family, which are required for the initiation and elongation of DNA replication. It has been reported that Cdc7/Dbf4 phospohrylates MCM2 during G1/S cell cycle which coincides with the intiation of DNA replication (3,4)

Images & Validation

• Tested Applications: FC, WB
• Dilution range: 1µg/mL - 0.001µg/mL. It is recommended that the reagent be titrated for optimal performance for each application. See product image legends for additional information.
• Reactivity: Human, Mouse, Rat

Key Properties

• Host: Rabbit
• Clonality: Monoclonal
• Isotype: Rabbit IgG1k
• Clone No.: MCM2S139-B12
• Immunogen: A synthetic phospho-peptide corresponding to residues surrounding Ser139 of human phospho MCM2
• Purification: Protein A+G
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 1X PBS, 0.02% NaN3, 50% Glycerol, 0.1% rAlbumin
• Concentration: 0.5 mg/mL
• Disclaimer: For research use only

Alternative Names

DNA replication licensing factor MCM2, Minichromosome maintenance protein 2 homolog, Nuclear protein BM28, CCNL1, CDCL1, KIAA0030

Flow cytometric analysis of C6 cells, secondary antibody only negative control (blue) or untreated (red) or treated with staurosporine (green) using Phospho-MCM2 (Ser139) antibody MCM2S139-B12 at 0.01 μg/mL. Cat. #orb1946023.

Western blot analysis of L929 cell extract untreated or treated with 25uM etoposide using Phospho-MCM2 (Ser139) antibody MCM2S139-B12 at 0.05 ug/mL. Cat. #orb1946023.

Peptide blocking flow cytometric analysis of C6 cells secondary antibody only negative control (light blue) or untreated (red) or treated with staurosporine (green) or untreated and blocked with phospho-peptide (black) or treated and blocked with phospho peptide (gold) or untreated and blocked with non-phospho peptide (dark blue) or treated and blocked with non-phospho peptide (purple) using Phospho-MCM2 (Ser139) antibody MCM2S139-B12 at 0.1 μg/mL. Cat. #orb1946023.

Western blot analysis of L929 cell extract untreated or treated with etoposide using 0.05 ug/mL Phospho-MCM2 (Ser139) antibody MCM2S139-B12. Cat.#orb1946023 or Company C antibody at 0.07 ug/mL (manufacturer's recommended concentration) developed using the same exposure.

Flow cytometric analysis of A431 cells, secondary antibody only negative control (blue), or untreated (grey) or treated with staurosporine (orange) using 0.01 ug/mL isotype control Cat. #orb1946241, or untreated (red) or treated (green) using Phospho-MCM2 (Ser139) antibody MCM2S139-B12 at 0.01 μg/mL. Cat. #orb1946023.

Quick Database Links

UniProt

P49736