Phospho-Lamin A/C (Ser22) rabbit mAb Antibody

SKU: orb1946019

Description

Lamins are nuclear membrane proteins that are involved with cell cycle control, chromatin organization, and DNA replication (1,2). Upon caspase 6 cleavage, lamin A/C serves as a molecular marker for capspase 6 activity. Lamin A/C is cleaved during cellular apoptosis into a large (41-50 kDa) as well as a small (28 kDa) fragment leading to nuclear dysfunction and cell death (3) Phosphorylation of lamin A/C at Ser32 by MAPK/CDK signals cell cycle progression and mitosis (4,5).

Research Area

Signal Transduction

Images & Validation

• Tested Applications: FC, WB
• Dilution Range: 1µg/mL - 0.001µg/mL. It is recommended that the reagent be titrated for optimal performance for each application. See product image legends for additional information.
• Reactivity: Human, Mouse

Key Properties

• Host: Rabbit
• Clonality: Monoclonal
• Isotype: Rabbit IgG1k
• Clone No.: BEH34
• Immunogen: A synthetic phospho-peptide corresponding to residues surrounding Ser22 of human phospho Lamin A/C
• Purification: Protein A+G
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 1X PBS, 0.02% NaN3, 50% Glycerol, 0.1% rAlbumin
• Concentration: 0.5 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Prelamin-A/C, 70 kDa lamin, Renal carcinoma antigen NY-REN-32, LMNA, LMN1

Flow cytometric analysis of HeLa cells untreated (red) or treated (green) using Phospho-Lamin A/C (Ser22) antibody LaminACS22-CF12 at 0.01 μg/mL. Cat. #orb1946019 or concentration matched isotope control for untreated (grey) or treated with nocodazole (orange) isotype control Cat. #orb1946241, or negative control (blue).

Western blot analysis of HeLa cell extract untreated or treated with nocodazole using Phospho-Lamin A/C (Ser22) antibody LaminACS22-CF12 at 10 ng/mL. Cat. #orb1946019.

Flow cytometric analysis of NIH 3T3 cells, secondary antibody only negative control (blue), or untreated (red) or treated with nocodazole (green) using Phospho-Lamin A/C (Ser22) antibody LaminACS22-CF12 at 0.05 μg/mL. Cat. #orb1946019.

Quick Database Links

UniProt

P02545