Phospho-KSR1 (Ser392) (3A4) rabbit mAb

• Catalog Number: orb1946217
• Category: Antibodies
• Description: Phospho-KSR1 (Ser392) (3A4) rabbit mAb
• Clonality: Monoclonal
• Clone Number: KSR1S392-3A4
• Tested applications: FC
• Reactivity: Human, Mouse
• Isotype: Rabbit IgGk
• Immunogen: A synthetic phospho-peptide corresponding to residues surrounding Ser392 of human phospho KSR1
• Concentration: 0.5 mg/ml
• Dilution range: 1µg/mL – 0.001µg/mL. It is recommended that the reagent be titrated for optimal performance for each application. See product image legends for additional information.
• Conjugation: Unconjugated
• UniProt ID: Q8IVT5
• Storage: -20°C
• Buffer/Preservatives: 1X PBS, 0.02% NaN3, 50% Glycerol, 0.1% rAlbumin
• Alternative names: Kinase suppressor of Ras 1
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

KSR1S392-3A4 recognizes basal phosphorylation levels in mouse cells. Flow cytometric analysis of NIH3T3 cells secondary antibody only (blue) or 0.1 ?μg/mL of isotype control Cat. #orb1946241 (orange) or of Phospho-KSR1 (S392) antibody KSR1S392-3A4 (green) Cat. #orb1946217.

Flow cytometric analysis of K562 cells secondary antibody only negative control (blue) treated with imatinib (red) treated with PV (green) treated with PV + blocked with non-phospho peptide (violet) or treated with PV + blocked with phospho-peptide (brown) using Phospho-KSR1 (S392) antibody KSR1S392-3A4 0.05 ?μg/mL. Cat. # orb1946217.

Flow cytometric analysis of HeLa human adenocarcinoma cells untreated and unstained as negative control (blue) or treated with lambda phosphatase and stained (red) or untreated and stained (green) using Phospho-KSR1 (S392) antibody, KSR1S392-3A4 at 0.1 μg/mL. Cat. #orb1946217.