Phospho-IRAK4 (Thr345) Rabbit Polyclonal Antibody

• Catalog Number: orb6226
• Category: Antibodies
• Description: Phospho-IRAK4 (Thr345) Rabbit Polyclonal Antibody
• Target: IRAK4
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Mouse
• Predicted Reactivity: Bovine, Canine, Guinea pig, Human, Porcine, Rabbit, Rat, Sheep
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Purification: Affinity purified by Protein A
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from human IRAK4 around the phosphorylation site of Thr345 VM(p-T)SR
• UniProt ID: Q9NWZ3
• RRID: AB_10921173
• MW: 51 kDa
• Tested applications: FC, WB
• Dilution range: WB=1:500-2000, Flow-Cyt=1ug/Test
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: IRAK4(phospho T345); p-IRAK4(phospho T345); IL-1 R
• Research Area: Cell Biology, Inflammation, Inflammatory Mediators
• Note: For research use only

Blank control (black line): NIH/3T3. Primary Antibody (green line): Rabbit Anti-phospho-IRAK4 (Thr345) antibody, dilution: 1 ug/Test, Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF488, dilution: 0.5 ug/Test. Isotype control (orange line): Normal Rabbit IgG, Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C, The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Sample: Lung (Mouse) Lysate at 40 ug, Primary: Anti-phospho-IRAK4 (Thr345) (orb6226) at 1/300 dilution, Secondary: HRP conjugated Goat-Anti-rabbit IgG (orb572747) at 1/5000 dilution, Predicted band size: 51 kD, Observed band size: 60 kD.