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Phospho-IKK gamma (Ser376) Rabbit Polyclonal Antibody

SKU: orb5529

Description

Phospho-IKK gamma (Ser376) Rabbit Polyclonal Antibody

Research Area

Signal Transduction

Images & Validation

Tested ApplicationsIF, IHC-Fr, IHC-P
Dilution RangeIHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
ReactivityRat
Predicted ReactivityHuman

Key Properties

Antibody TypePrimary Antibody
HostRabbit
ClonalityPolyclonal
IsotypeIgG
ImmunogenKLH conjugated Synthesised phosphopeptide derived from human IKBKG around the phosphorylation site of Ser376 YL(p-S)SP-NH2
TargetIKBKG
Molecular Weight46 kDa
PurificationAffinity purified by Protein A
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/AppearanceLiquid
Buffer/Preservatives0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration1mg/ml
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

IKBKG | IKK gamma (p-S376); p-IKK gamma; phospho-IKK gamma; AMCBX1; EDAID1; FIP-3; FIP3; Fip3p; IKK-gamma; IKKAP1; IKKG; IMD33; IP; IP1; IP2; IPD2; NEMO; SAIDX; ZC2HC9; 1110037D23Rik; IKK[g]; NEMO_HUMAN; IKBKG; IkB kinase-associated protein 1 (IKKAP1); Inhibitor of nuclear factor kappa-B kinase subunit gamma (I-kappa-B kinase subunit gamma | IKK-gamma | IKKG | IkB kinase subunit gamma); NF-kappa-B essential modifier; NEMO_MOUSE; IkB kinase-associated protein 1 (IKKAP1 | mFIP-3); NEMO_RAT;

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Phospho-IKK gamma (Ser376) Rabbit Polyclonal Antibody

Tissue/Cell: rat brain tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-phospho-IKK gamma (Ser376) Polyclonal Antibody, Unconjugated (orb5529) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

UniProt Details

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Protocol Information

IHC-P
Immunohistochemistry Paraffin
View Protocol
IHC-Fr
Immunohistochemistry Frozen
View Protocol
IF
Immunofluorescence
View Protocol

Phospho-IKK gamma (Ser376) Rabbit Polyclonal Antibody (orb5529)

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50 μl
$ 250.00
100 μl
$ 390.00
200 μl
$ 470.00
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