Phospho-IKK gamma (Ser31) Rabbit Polyclonal Antibody

SKU: orb5528

Description

Phospho-IKK gamma (Ser31) Rabbit Polyclonal Antibody

Research Area

Signal Transduction

Images & Validation

• Tested Applications: FC, IF, IHC-Fr, IHC-P
• Dilution Range: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1ug/Test
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from human IKK gamma around the phosphorylation site of Ser31 EE(p-S)PL
• Target: IKBKG
• Molecular Weight: 46 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

IKBKG | IKK gamma (p-S31); p-IKK gamma; phospho-IKK gamma; AMCBX1; EDAID1; FIP-3; FIP3; Fip3p; IKK-gamma; IKKAP1; IKKG; IMD33; IP; IP1; IP2; IPD2; NEMO; SAIDX; ZC2HC9; 1110037D23Rik; IKK[g]; NEMO_HUMAN; IKBKG; IkB kinase-associated protein 1 (IKKAP1); Inhibitor of nuclear factor kappa-B kinase subunit gamma (I-kappa-B kinase subunit gamma | IKK-gamma | IKKG | IkB kinase subunit gamma); NF-kappa-B essential modifier; NEMO_MOUSE; IkB kinase-associated protein 1 (IKKAP1 | mFIP-3); NEMO_RAT;

Paraformaldehyde-fixed, paraffin embedded (Human liver carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (phospho-IKK gamma (Ser31)) Polyclonal Antibody, Unconjugated (orb5528) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Blank control (black line): Hela. Primary Antibody (green line): Rabbit Anti-phospho-IKK gamma (Ser31) antibody (orb5528), dilution: 1 ug/Test, Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC, dilution: 0.5 ug/Test. Isotype control (orange line): Normal Rabbit IgG, Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C, The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Quick Database Links

Gene Symbol

IKBKG

UniProt

Q9Y6K9