Phospho-IKB Alpha (Ser32 / Ser36) Recombinant Rabbit Monoclonal Antibody

• Catalog Number: orb1499271
• Category: Antibodies
• Description: Phospho-IKB Alpha (Ser32 / Ser36) Recombinant Rabbit Monoclonal Antibody
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: R8D3
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Human, Mouse, Rat
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human phospho-IKB Alpha (Ser32 / Ser36)
• Antibody Type: Recombinant Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:50-100, IF=1:1:50-100
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 35 kDa
• Target: NFKBIA
• UniProt ID: P25963
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: NFKBIA; Inhibitor of KB alpha; I kappa B alpha; I(
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Blocking buffer: 5% NFDM/TBST, Primary Ab Dilution: 1:2000, Primary Ab incubation condition: 2 hours at room temperature, Secondary Ab: Goat Anti-Rabbit IgG H&L (HRP), Lysate: (-): C6, (+): C6 + Calyculin A (100nM, 30 min), Protein loading quantity: 20 µg, Exposure time: 60 s, Predicted MW: 36 kDa, Observed MW: 36 kDa.

Blocking buffer: 5% NFDM/TBST, Primary Ab Dilution: 1:2000, Primary Ab incubation condition: 2 hours at room temperature, Secondary Ab: Goat Anti-Rabbit IgG H&L (HRP), Lysate: (-): HeLa, (+): HeLa + Human α-TNF, (20 ng/ml, 30 min), Protein loading quantity: 20 µg, Exposure time: 30 s, Predicted MW: 36 kDa, Observed MW: 36 kDa.

Tissue: (A) Mouse kidney, (B) Mouse kidney, then the tissue was incubated with alkaline, phosphatase (50 U/ml) at 37°C for 1 hour, Section type: Formalin-fixed & Paraffin-embedded section, Retrieval method: High temperature and high pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0, Primary Ab Dilution: 1:100, Primary Ab incubation condition: 1 hour at room temperature, Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to use), Counter stain: Hematoxylin (Blue), Comment: Color brown is the positive signal for orb1499271.

Tissue: (A) Rat kidney, (B) Rat kidney, then the tissue was incubated with alkaline phosphatase (50, U/ml) at 37°C for 1 hour, Section type: Formalin-fixed & Paraffin-embedded section, Retrieval method: High temperature and high pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0, Primary Ab Dilution: 1:100, Primary Ab incubation condition: 1 hour at room temperature, Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to use), Counter stain: Hematoxylin (Blue), Comment: Color brown is the positive signal for orb1499271.