Phospho-HS1 (Tyr397) (F12) rabbit mAb Antibody

• Catalog Number: orb1946044
• Category: Antibodies
• Description: Phospho-HS1 (Tyr397) (F12) rabbit mAb Antibody
• Clonality: Monoclonal
• Clone Number: HS1Y397-F12
• Tested applications: FC, WB
• Reactivity: Human, Mouse
• Isotype: Rabbit IgGk
• Immunogen: A synthetic phospho-peptide corresponding to residues surrounding Tyr397 of human phospho HS1
• Concentration: 0.5 mg/ml
• Dilution range: 1µg/mL – 0.001µg/mL. It is recommended that the reagent be titrated for optimal performance for each application. See product image legends for additional information.
• Conjugation: Unconjugated
• UniProt ID: P14317
• Storage: -20°C
• Buffer/Preservatives: 1X PBS, 0.02% NaN3, 50% Glycerol, 0.1% rAlbumin
• Alternative names: Hematopoietic lineage cell-specific protein, Hemat
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Flow cytometric analysis of Ramos cells secondary antibody only negative control (blue) or untreated (red) or treated with pervanadate (green) using Phospho-HS1 (Tyr397) antibody HS1Y397-F12 at 0.01μg/mL. Cat. #orb1946044.

Western blot analysis of Ramos cell extract, untreated or treated with 300 nM Thapsigargin for 30 min using HS1 (Tyr397) antibody HS1Y397-F12 at 0.01 μg/mL. Cat. #orb1946044.

Peptide blocking flow cytometric analysis of Ramos cells secondary antibody only negative control (light blue) or untreated (red) or treated with pervandadate (green) or untreated and blocked with phospho-peptide (black) or treated and blocked with phospho peptide (gold) or untreated and blocked with non-phospho peptide (dark blue) or treated and blocked with non-phospho peptide (purple) using Phospho-HS1 (Tyr397) antibody HS1Y397-F12 at 0.01μg/mL. Cat. #orb1946044.

Western blot analysis of Ramos cell extract untreated or treated with 300 nM thapsigargin for 30 min using 10 ng/mL Phospho-HS1 (Tyr397) antibody HS1Y397-F12 at 0.01μg/mL. Cat. #orb1946044 or Company C antibody at 3 ng/mL (manufacturer's recommended concentration) developed using the same exposure.

Flow cytometric analysis of mouse cells secondary antibody only negative control (blue) or control (red) or stimulated (green) using Phospho-HS1 (Tyr397) antibody HS1Y397-F12 at 0.01μg/mL. Cat. #orb1946044.