Phospho-Histone H3 (Thr80) Rabbit Polyclonal Antibody

• Catalog Number: orb1172878
• Category: Antibodies
• Description: Phospho-Histone H3 (Thr80) Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: WB
• Predicted Reactivity: Mouse, Rat
• Reactivity: Human
• Isotype: IgG
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-1000
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: H3C1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Blocking buffer: 5% NFDM/TBST, Primary ab Dilution: 1:2000, Primary ab incubation condition: 2 hours at room temperature, Secondary ab: Goat Anti-Rabbit IgG H&L (HRP), Lysate: (-) HeLa, (+) HeLa + Okadaic acid (100nM, 1hr) + Calyculin A (100nM, 1hr), Protein loading quantity: 20 µg, Exposure time: 60 s, Predicted MW: 17 kDa, Observed MW: 17 kDa.

Blocking buffer: 5% NFDM/TBST, Primary ab Dilution: 1:2000, Primary ab incubation condition: 2 hours at room temperature, Secondary ab: Goat Anti-Rabbit IgG H&L (HRP), Lysate: Mouse brain, Mouse testis, MCF-7, Protein loading quantity: 20 µg, Exposure time: 60 s, Predicted MW: 17 kDa, Observed MW: 17 kDa.