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Description
Research Area
Epigenetics & Chromatin
Images & Validation
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Item 1 of 4
| Tested Applications | ICC, IF, WB |
|---|---|
| Dilution Range | WB: 1/500-1/1000 IF: 1/50-1/200 |
| Reactivity | Human, Mouse |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG2a |
| Clone No. | B4C1XD7 |
| Immunogen | Synthetic phosphopeptide corresponding to residues surrounding Ser139 of human H2A.X. |
| Target | H2AX |
| Molecular Weight | Calculated MW: 15 kDa; Observed MW: 15 kDa |
| Purification | Affinity Purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid in PBS containing 50% glycerol, 0.5% rAlbumin and 0.02% sodium azide, pH 7.3. |
| Buffer/Preservatives | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Concentration | 6.67 mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−H2A.X; H2AFX; H2a/x; HIST5-2AX; Histone H2A.X; gamma H2A.X
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Quick Database Links
Gene Symbol
H2AX
UniProt
UniProt Details
− No UniProt data available
Protocol Information
WB
Western Blot (IB, immunoblot)
IF
Immunofluorescence
ICC
Immunocytochemistry
Available Sizes
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