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Phospho-Histone H2A.X (Ser139) (7G9) Mouse mAb
Description
Images & Validation
−| Tested Applications | ICC, IF, WB |
|---|---|
| Dilution range | WB: 1/500-1/1000 IF: 1/50-1/200 |
| Reactivity | Human, Mouse |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone No. | 7G9-H4 |
| Immunogen | Synthetic phosphopeptide corresponding to residues surrounding Ser139 of human H2A.X. |
| Target | H2AX |
| Molecular Weight | Calculated MW: 15 kDa; Observed MW: 15 kDa |
| Purification | Affinity Purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | Liquid in PBS containing 50% glycerol, 0.5% rAlbumin and 0.02% sodium azide, pH 7.3. |
| Concentration | 3.23 mg/ml |
| Disclaimer | For research use only |
Alternative Names
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Western blot analysis of Phosphorylation of H2A.X at Serine 139 in 3T3 or Hydroxyureatreated 3T3 lysates using Phospho-Histone H2A.X (Ser139) antibody.

Immunofluorescence analysis of Phospho-Histone H2A.X (Ser139) (7G9) in 3T3 or Hydroxyureatreated 3T3 using Phospho-Histone H2A.X (Ser139) antibody.

Immunofluorescence analysis of Phospho-Histone H2A.X (Ser139) (7G9) in A549 (upper, untreated or Hydroxyureatreated) and Hela (lower, untreated or Hydroxyureatreated) using Phospho-Histone H2A.X (Ser139) antibody.
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UniProt Details
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Protocol Information
Phospho-Histone H2A.X (Ser139) (7G9) Mouse mAb (orb1563162)
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