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Phospho-Histone H2A.X (Ser139) (7G9) Mouse mAb

SKU: orb1563162
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Description

Phospho-Histone H2A.X (Ser139) (7G9) Mouse mAb

Images & Validation

Tested ApplicationsICC, IF, WB
Dilution rangeWB: 1/500-1/1000 IF: 1/50-1/200
ReactivityHuman, Mouse

Key Properties

Antibody TypePrimary Antibody
ClonalityMonoclonal
IsotypeIgG1
Clone No.7G9-H4
ImmunogenSynthetic phosphopeptide corresponding to residues surrounding Ser139 of human H2A.X.
TargetH2AX
Molecular WeightCalculated MW: 15 kDa; Observed MW: 15 kDa
PurificationAffinity Purified
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/AppearanceLiquid
Buffer/PreservativesLiquid in PBS containing 50% glycerol, 0.5% rAlbumin and 0.02% sodium azide, pH 7.3.
Concentration3.23 mg/ml
DisclaimerFor research use only

Alternative Names

H2A.X; H2AFX; H2a/x; HIST5-2AX; Histone H2A.X; gamma H2A.X
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Phospho-Histone H2A.X (Ser139) (7G9) Mouse mAb

Western blot analysis of Phosphorylation of H2A.X at Serine 139 in 3T3 or Hydroxyureatreated 3T3 lysates using Phospho-Histone H2A.X (Ser139) antibody.

Phospho-Histone H2A.X (Ser139) (7G9) Mouse mAb

Immunofluorescence analysis of Phospho-Histone H2A.X (Ser139) (7G9) in 3T3 or Hydroxyureatreated 3T3 using Phospho-Histone H2A.X (Ser139) antibody.

Phospho-Histone H2A.X (Ser139) (7G9) Mouse mAb

Immunofluorescence analysis of Phospho-Histone H2A.X (Ser139) (7G9) in A549 (upper, untreated or Hydroxyureatreated) and Hela (lower, untreated or Hydroxyureatreated) using Phospho-Histone H2A.X (Ser139) antibody.

UniProt Details

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Protocol Information

WB
Western Blot (IB, immunoblot)
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IF
Immunofluorescence
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ICC
Immunocytochemistry
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Phospho-Histone H2A.X (Ser139) (7G9) Mouse mAb (orb1563162)

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Available Sizes

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20 μl
$ 120.00
50 μl
$ 210.00
100 μl
$ 350.00
DispatchUsually dispatched within 5-10 working days
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