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Item 1 of 3
Phospho-H2AFX (S139) Antibody
Catalog Number: orb416660
| Catalog Number | orb416660 |
|---|---|
| Category | Antibodies |
| Description | Phospho-H2AFX (S139) Antibody |
| Target | H2AFX |
| Clonality | Polyclonal |
| Species/Host | Rabbit |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Reactivity | Human |
| Form/Appearance | Liquid |
| Buffer/Preservatives | Preservative: 0.03% Proclin 300. Constituents: 50% Glycerol |
| Immunogen | Peptide sequence around site of Phospho-Ser (139) derived from Human Histone H2AX |
| UniProt ID | P16104 |
| Tested applications | ChIP, ELISA, IF, WB |
| Dilution range | WB:1:500-5000, IF:1:50-200 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Alternative names | Anti-Histone H2AX antibody, anti- H2a/x antibody, Read more... |
| Note | For research use only |
Chromatin Immunoprecipitation Hela (4*106) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5ug anti- H2AFX (phospho-S139) antibody or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-t
Western blot aanalysis of Hela whole cell lysate, 293 whole cell lysate, K562 whole cell lysate, HepG2 whole cell lysateusing H2AFX (phospho-S139) antibody
Immunofluorescence staining of Hela using H2AFX (phospho-S139) antibody
