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Phospho-GSK3 Alpha + Beta (Tyr279+Tyr216) Recombinant Rabbit Monoclonal Antibody
Catalog Number: orb783424
| Catalog Number | orb783424 |
|---|---|
| Category | Antibodies |
| Description | Phospho-GSK3 Alpha + Beta (Tyr279+Tyr216) Recombinant Rabbit Monoclonal Antibody |
| Species/Host | Rabbit |
| Clonality | Recombinant |
| Clone Number | 5G8 |
| Tested applications | ICC, IF, IHC-Fr, IHC-P, WB |
| Predicted Reactivity | Mouse, Rat |
| Reactivity | Human, Mouse, Rat |
| Isotype | IgG |
| Immunogen | KLH conjugated Synthesised phosphopeptide derived from human GSK-3 Beta around the phosphorylation site of Tyr216 |
| Antibody Type | Recombinant Antibody |
| Concentration | 1mg/ml |
| Dilution range | WB=1:200-1000, IHC-P=1:20-100, IHC-F=1:400-800, ICC/IF=1:20-50, IF=1:100-500 |
| Form/Appearance | Liquid |
| Conjugation | Unconjugated |
| MW | 47/51 kDa |
| Target | GSK3B |
| UniProt ID | P49841 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Alternative names | phospho-GSK3(alpha+beta)(Y216+Y279); GSK3B(phospho Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
Hela cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (Phospho-GSK3 (alpha+beta) (Y216+Y279)) monoclonal Antibody, Unconjugated (orb783424) 1:50, 90 minutes at 37°C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.
Paraformaldehyde-fixed, paraffin embedded (human breast carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (phospho-GSK3 Alpha + Beta (Tyr279+Tyr216)) Monoclonal Antibody, Unconjugated (orb783424) at 1:50 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (phospho-GSK3 Alpha + Beta (Tyr279+Tyr216)) Monoclonal Antibody, Unconjugated (orb783424) at 1:50 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (phospho-GSK3 Alpha + Beta (Tyr279+Tyr216)) Monoclonal Antibody, Unconjugated (orb783424) at 1:50 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Sample: Lane 1: Mouse Cerebrum tissue lysates, Lane 2: Mouse Testis tissue lysates, Lane 3: Rat Cerebrum tissue lysates, Lane 4: Rat Testis tissue lysates, Lane 5: Human HeLa cell lysates, Lane 6: Human 293T cell lysates, Lane 7: Human HepG2 cell lysates, Lane 8: Human MCF-7 cell lysates, Primary: Anti-phospho-GSK3 Alpha + Beta (Tyr279+Tyr216) (orb783424) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 47/51 kDa, Observed band size: 47, 51 kDa.
