Phospho-EGFR(Y1172) Antibody

• Catalog Number: orb1931173
• Category: Antibodies
• Description: Phospho-EGFR(Y1172) Antibody
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• UniProt ID: P00533
• MW: 134277 Da
• Tested applications: DOT, WB
• Dilution range: WB - 1:1000, DB - 1:500
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: ERBB, ERBB1, HER1
• Research Area: Cancer Biology, Signal Transduction
• Note: For research use only

Dot blot analysis of anti-EGFR-pY1172 Phospho-specific Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.

Western blot analysis of EGFR (arrow) in Hela cell lysates, either induced (Lane 1) or noninduced with EGF (Lane 2).

Western blot analysis of extracts from A431 cells, untreated or treated with EGF, using phospho EGFR-Y1172 (left) or ErBB2 antibody (right)