Phospho-EGFR(Y1069) Antibody

• Catalog Number: orb1931203
• Category: Antibodies
• Description: Phospho-EGFR(Y1069) Antibody
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• UniProt ID: P00533
• MW: 134277 Da
• Tested applications: DOT, WB
• Dilution range: WB - 1:2000, DB - 1:500
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: ERBB, ERBB1, HER1
• Research Area: Cancer Biology, Signal Transduction
• Note: For research use only

(LEFT) Dot blot analysis of Phospho-EGFR-Y1069 polyclonal antibody on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentration was 0.5ug per ml. P-Pab: phospho-antibody; P-Peptide: phospho-peptide; NP-Peptide: non-phospho-peptide. (RIGHT) Western blot analysis of EGFRY (arrow) in Hela cell lysates, either noninduced (Lane 1) or induced with EGF (Lane 2).

Western blot analysis of lysates from A431 cell line, untreated or treated with EGF, 100ng/ml, using EGFRY Antibody (p1069) (upper) or EGFR (lower).