Phospho-EGFR(Y1016) Antibody

• Catalog Number: orb1931130
• Category: Antibodies
• Description: Phospho-EGFR(Y1016) Antibody
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Mouse
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• UniProt ID: P00533
• MW: 134277 Da
• Tested applications: DOT, WB
• Dilution range: WB - 1:8000, DB - 1:500
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: ERBB, ERBB1, HER1
• Research Area: Cancer Biology, Signal Transduction
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Dot blot analysis of anti-EGFR B1 Phospho-specific Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.

Western blot analysis of lysate from A431 cells (from left to right), untreated or treated with EGF at 100ng/ml, using Phospho-EGFR-pY1016 Antibody or EGFR-pS695 Antibody. Lysate at 15ug per lane.Diluted at 1: 8000 dilution at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody.